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Monoclonal Mouse Anti-Human
CD30
Clone Ber-H2
CD30 expression is found on Hodgkin's and Reed-Sternberg cells, and on activted B and T lymphocytes. CD30 is also expressed by embryonal carcinoma cells.
This antibody is a useful tool for the identification of anaplastic large cell lymphoma and Hodgkin's disease.
References
- Schwarting R, Gerdes J, Dürkop H, Falini B, Pileri S, Stein H. Ber-H2: a new anti-Ki-1 (CD30) monoclonal antibody directed at a formol-resistant epitope. Blood 1989;74:1678-89.
Hodgkin's lymphoma (FFPE) stained with FLEX Anti-CD30, Code IR602/IS602.
Classic Hodgkin lymphoma. The majority of the Reed-Sternberg cells show a distinct membranous staining reaction and a paranuclear dot-like reaction.
Clinical application for IR602/IS602. For identification of Reed-Sternberg cells in Hodgkin lymphoma and anaplastic large cell lymphoma.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human CD30, Clone Ber-H2, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels anaplastic large-cell lymphoma (ALCL) and Reed-Sternberg cells, and is useful for the identification of ALCL and as a secondary marker for Hodgkin's disease (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human CD30, Clone Ber-H2, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels anaplastic large-cell lymphoma (ALCL) and Reed-Sternberg cells, and is useful for the identification of ALCL and as a secondary marker for Hodgkin's disease (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Co cell line established from a patient with Hodgkin's disease of T-cell lineage (1, 6).
Specificity
Anti-Human CD30, clone Ber-H2, was clustered as anti-CD30 at the Fourth International Workshop and Conference on Human Leucocyte Differentiation Antigens (7).
SDS-PAGE analysis of immunoprecipitates formed between lysate of 125I-labeled COS cells transfected with cDNA encoding CD30 and the antibody shows reaction with a 120 kDa protein corresponding to CD30. Mock-transfected COS cells were negative. The epitope recognized by the antibody is located between amino acid residues 112 and 412 (8).
The antibody labels cell lines derived from Hodgkin's disease, L428, L540, L591, Co, Ho and KM-H2; HTLV-1 transfected T-cell lines, Hut-102 and MT-2; EBV-transformed B cell lines (non-Burkitt), B95-8 (monkey), BJA-B and Cess; and the myeloid cell line, K 562 (1).
SDS-PAGE analysis of immunoprecipitates formed between lysate of 125I-labeled COS cells transfected with cDNA encoding CD30 and the antibody shows reaction with a 120 kDa protein corresponding to CD30. Mock-transfected COS cells were negative. The epitope recognized by the antibody is located between amino acid residues 112 and 412 (8).
The antibody labels cell lines derived from Hodgkin's disease, L428, L540, L591, Co, Ho and KM-H2; HTLV-1 transfected T-cell lines, Hut-102 and MT-2; EBV-transformed B cell lines (non-Burkitt), B95-8 (monkey), BJA-B and Cess; and the myeloid cell line, K 562 (1).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: Ber-H2 (1). Isotype: IgG1, kappa.
Clone: Ber-H2 (1). Isotype: IgG1, kappa.
References
- Schwarting R, Gerdes J, Dürkop H, Falini B, Pileri S, Stein H. Ber-H2: A new anti-Ki-1 (CD30) monoclonal antibody directed at a formol-resistant epitope. Blood 1989;74:1678-89.
- Drexler HG, Minowada J. Hodgkin's disease derived cell lines: a review [review]. Hum Cell 1992;5:42-53.
- Schwarting R, Stein H. A3. Cluster report: CD30. In: Knapp W, Dörken B, Gilks WR, Rieber EP, Schmidt RE, Stein H, et al., editors. Leucocyte typing IV. White cell differentiation antigens. Proceedings of the 4th International Workshop and Conference; 1989 Feb 21-25; Vienna, Austria. Oxford, New York, Tokyo: Oxford University Press; 1989. p. 419-22.
- Dürkop H, Latza U, Hummel M, Eitelbach F, Seed B, Stein H. Molecular cloning and expression of a new member of the nerve growth factor receptor family that is characteristic for Hodgkin's disease. Cell 1992;68:421-7.
| Product | Size |  | IS602 Clone Ber-H2
| 30 tests, 6 mL Product No. IS60230 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, Low pH (Code K8005) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800521 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Tonsil | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use Low pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
Synonym(s) for Antigen
Ki-1 antigen