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Polyclonal Rabbit Anti-
Herpes Simplex Virus Type 1
Herpes simplex virus (HSV) belongs to the Herpesviridae family of viruses. HSV infects cells of the mucoepithelia or the skin.
The antibody reacts with type-specific, i.e. HSV-1, as well as with type-common, i.e. also with HSV-2, antigens. This antibody is useful for the identification of herpes simplex virus in human cellular material.
References
- Schmidt NJ, Dennis J, Devlin V, Gallo D, Mills J. Comparison of direct immunofluorescence and direct immunoperoxidase procedures for detection of herpes simplex virus antigen in lesion specimens. J Clin Microbiol 1983;18:445-8.
- Miller MJ, Howell CL. Rapid detection and identification of herpes simplex virus in cell culture by a direct immunoperoxidase staining procedure. J Clin Microbiol 1983;18:550-3.
- Vestergaard BF, Grauballe PC. ELISA for herpes simplex virus (HSV) type-specific antibodies in human sera using HSV type 1 and type 2 polyspecific antigens blocked with type-heterologous rabbit antibodies. Acta path microbiol scand Sect. B 1979;87:261-3.
Liver (FFPE) stained with FLEX Anti-Herpes Simplex Virus Type 1, Code IR521/IS521.
Liver. Cells infected with herpes simplex virus show a weak to strong and occasionally diffuse intranuclear staining reaction.
Clinical application for IR521/IS521 For identification of herpes simplex virus infection in human tissue.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Herpes Simplex Virus Type 1, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. The antibody reacts with type-specific as well as with type-common antigens. This antibody is useful for theidentification of herpes simplex virus in human cellular material (1, 2). The clinical interpretation of any staining or its absence should becomplemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Polyclonal Rabbit Anti-Herpes Simplex Virus Type 1, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. The antibody reacts with type-specific as well as with type-common antigens. This antibody is useful for theidentification of herpes simplex virus in human cellular material (1, 2). The clinical interpretation of any staining or its absence should becomplemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Detergent-solubilized HSV type 1 (strain MacIntyre)-infected whole rabbit cornea cells (continuous cell line).
Specificity
The antibody reacts with HSV type 1 specific antigens and with antigens common for HSV types 1 and 2. The antibody reacts with all the majorglycoproteins present in the viral envelope and with at least one core protein as determined by crossed immunoelectrophoresis. Contaminating antibodies to human and bovine serum have been removed by solid-phase absorption.
In ELISA the antibody shows no reaction with human and bovine plasma. The antibody shows no cross-reactivity to cytomegalovirus andEpstein-Barr virus.
In indirect immunofluorescence a weak cross-reactivity to varicella zoster virus can be expected if the antibody is used in a relatively highconcentration. However, no cross-reactivity was found when the antibody was tested at a dilution of 1:100.
In ELISA the antibody shows no reaction with human and bovine plasma. The antibody shows no cross-reactivity to cytomegalovirus andEpstein-Barr virus.
In indirect immunofluorescence a weak cross-reactivity to varicella zoster virus can be expected if the antibody is used in a relatively highconcentration. However, no cross-reactivity was found when the antibody was tested at a dilution of 1:100.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
References
- Debiasi RL, Kleinschmidt-DeMasters BK, Richardson-Burns S, Tyler KL. Central nervous system apoptosis in human herpes simpex virus and cytolomegavirus encephalitis. J Infect Dis 2002;186:1547-57.
- Itoh T, Takahashi T, Kusaka K, Kawaura K, Nakagawa Y, Yamakawa J, et al. Herpes simplex esophagitis from 1307 autopsy cases. J Gastroenterol Hepatol 2003;18:1407-11.
| Product | Size |  | IS521
| 30 tests, 6 mL Product No. IS52130 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IS600) Positive tissue: A lesion with HSV infection | 60 tests, 12 mL Product No. IS60061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
Synonym(s) for Antigen
HSV, HSV-1, HSV1