Dako Kappa Lambda Study Findings

Evaluation of Commercial Kappa/Lambda Reagents for Flow Cytometric Analysis of Normal and Abnormal Specimens

J. Lemenager and B.K. DuChateau

Abstract:

The evaluation of cell surface Kappa/Lambda expression can identify clonally restricted B lymphocyte populations and thus can aide in the diagnosis of hematologic malignancy. Several B cell disorders are associated with decreased levels of Kappa/Lambda at the cell surface. In these cases, it is important to utilize Kappa/Lambda reagents that can optimally identify Kappa/Lambda cell surface expression even when decreased levels are present. In this study, flow cytometric analysis was used to evaluate the performance of Kappa/Lambda reagents from two different commercial vendors (Becton Dickinson, and Dako). Normal specimens and abnormal specimens with decreased levels of Kappa/Lambda cell surface expression were interrogated in the study. Mean channel fluorescence (MCF) and Signal to noise ratios (S:N) of Kappa and Lambda expression for all reagents were collected and compared. The Dako reagents demonstrated an increased MCF and S:N of both Kappa and Lambda expression compared with the Becton Dickinson reagents when normal peripheral blood specimens were analyzed (P <0.05). Abnormal specimens with decreased levels of Kappa at the cell surface were also analyzed. Again, The Dako reagents demonstrated an increased MCF and S:N compared with the Becton Dickinson reagents (P <0.05). Dako Kappa and Lambda reagents are superior to Becton-Dickinson in detecting cell surface expressed Kappa and Lambda especially when decreased levels are present.

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