Immunohistochemical Staining Methods Education Guide

Dako is pleased to present the 6th edition of Immunohistochemical Staining Methods. The updated guidebook is intended to help drive further scientific advancement and increase standardization in the field of immunohistochemistry.

Download the full Immunohistochemical Staining Methods Education Guide - Sixth Edition

Order a copy or contact your local Dako office for inquiries on the hardback edition of the guidebook.

IHC Guidebook: Immunohistochemical Staining Methods

Download individual chapters from the IHC Guidebook

Preface - IHC Guidebook . Immunohistochemical Staining Methods

Innovation and Quality | Preface

Welcome to the Sixth Edition of Dako’s Guidebook to Immunohistochemical Staining Methods.

Innovation and Quality | Preface

Chapter 1 - Introduction to Immunohistochemistry

Introduction to Immunohistochemistry
| Chapter 1

Chapter 1 gives an overview of immunohistochemistry (IHC) as it is used in the pathology laboratory to determine the expression of biomarkers in tissue.

Introduction to Immunohistochemistry | Chapter 1

Chapter 2 - Fixation and Other Pre-Analytical Factors

Fixation and Other Pre-Analytical Factors
| Chapter 2

This chapter will address some of the pre-analytical issues that should be considered when preparing sections for IHC for accurate detection of targets.

Fixation and Other Pre-Analytical Factors | Chapter 2

Chapter 3 - Antigen Retrieval

Antigen Retrieval | Chapter 3

Formalin-fixation of tissue may cause loss of the ability of the antibody to react with the antigen. This chapter describes the process of antigen retrieval.

Antigen Retrieval | Chapter 3

Chapter 4 - Selection of the Primary Antibody

Selection of the Primary Antibody
| Chapter 4

This chapter will focus on the considerations to which users must pay special attention when selecting the right antibody for a given IHC test.

Selection of the Primary Antibody | Chapter 4

Chapter 5 - Staining Protocol Optimization

Staining Protocol Optimization | Chapter 5

This chapter describes the requirements for an optimal staining protocol in each step from antigen retrieval to visual chromogenic deposition.

Staining Protocol Optimization | Chapter 5

Chapter 6 - Detection Methods

Detection Methods | Chapter 6

Visualization of targets in tissue with high sensitivity and low background using chromogen, immunofluorescence and multistain technologies are explained here.

Detection Methods | Chapter 6

Chapter 7 - Digital Pathology

Digital Pathology | Chapter 7

Many pathologists are still examining glass slides at a bright field microscope. However, new computer-based technologies offer the real prospect of radical change.

Digital Pathology | Chapter 7

Chapter 8 - Optimization of Immunohistochemical Reactions

Optimization of Immunohistochemical Reactions | Chapter 8

This chapter will discuss the formulation and use of several basic immunohistochemical reagents as they relate to assay optimization.

Optimization of Immunohistochemical Reactions | Chapter 8

Chapter 9 - Automation in IHC

Automation in IHC | Chapter 9

The different IHC staining principles being used on commercially available instrument systems and the influence of automation on standardization are explored.

Automation in IHC | Chapter 9

Chapter 10 - Optimizing Laboratory Workflow

Optimizing Laboratory Workflow
| Chapter 10

Workflow must be designed around solutions that respect and exploit the unique characteristics and needs of each operation. After all, no two labs are the same.

Optimizing Laboratory Workflow | Chapter 10

Chapter 11 - Companion Diagnostics

Companion Diagnostics | Chapter 11

The development pathway of a companion diagnostics assay used to select patients for treatment decisions is clarified in this chapter.

Companion Diagnostics | Chapter 11

Chapter 12 - Tissue Microarray – Construction and QA

Tissue Microarray – Construction and QA
| Chapter 12

TMAs allow performance of IHC assays on many samples in an efficient and cost-effective manner. Some of the theoretical and practical approaches are considered here.

Tissue Microarray – Construction and QA | Chapter 12

Chapter 13 - Immunohistochemical Visualization of Molecular Tests

Immunohistochemical Visualization of Molecular Tests | Chapter 13

This chapter focuses on the immunohistochemical aspects of molecular testing for genetic aberrations in cancer using CISH as example.

Immunohistochemical Visualization of Molecular Tests | Chapter 13

Chapter 14 - Controls

Controls | Chapter 14

Controls are used for verification of IHC results for in vitro diagnostic use. It is important to understand what information a given control can and cannot provide.

Controls | Chapter 14

Chapter 15 - Background

Background | Chapter 15

This chapter will cover the main causes of background staining related to antibodies, detection methods, and other general factors and will offer possible solutions.

Background | Chapter 15

Chapter 16 - Background

Troubleshooting | Chapter 16

The information contained in this chapter should enable the user rapidly to pinpoint and resolve problems encountered during the staining procedure.

Troubleshooting | Chapter 16

Appendix A - Antibodies

Antibodies | Appendix A

Appendix A briefly covers the structural and biochemical features of antibodies and antigens and is as such not a necessity to understand the IHC staining procedure.

Antibodies | Appendix A

Appendix B - Basic Immunochemistry

Basic Immunochemistry | Appendix B

Appendix B will briefly describe how changes to titer, dilution, incubation time and temperature may influence the staining reaction.

Basic Immunochemistry | Appendix B

Please contact your local Dako office for inquiries on the hardback edition of the guidebook.

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