Blotting Detection System
Chemiluminescent System for Nucleic Acid Blotting is a complete kit for the detection of Southern or Northern blot hybridizations using fluorescein-labeled probes. Hybridized probes are detected by incubation with an alkaline phosphatase-conjugated antibody fragment directed against fluorescein. The immobilized alkaline phosphatase activity is used to hydrolyze a chemiluminescent dioxetane-phosphate substrate, resulting in sustained light emission at the site of the hybridized probe. The light emitted from the blot is captured by overlaying the blot with photographic film, or by imaging using a sensitive CCD camera system.
Special features of the Dako Chemiluminescent System:
- Non-radioactive reagents (health and disposal issues eliminated)
- Exposure time reduced to minutes as opposed to days using the 32P-method
- Complete with all necessary detection reagents in either liquid concentrates or pre-measured powder packets for convenience
- Uses standard film and processing equipment