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Monoclonal Mouse Anti-Human
Cytokeratin 18
Clone DC 10
Reacts with the 45 kDa protein corresponding to cytokeratin 18. The antibody labels a large number of simple epithelia, including many ductal and glandular epithelia.
The antibody labels tumor cells of epithelial origin, whereas tumor cells of non-epithelial origin such as glioma, melanoma and osteosarcoma, are not labeled. In vascular tumors, the antibody labels endothelial cells in epithelioid hemangioendotheliomas.
- Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 1988;7:495-504.
Merkel cell carcinoma (FFPE) stained with FLEX Anti-Cytokeratin 18, Code IR618/IS618.
Merkel cell carcinoma. The majority of the neoplastic cells show a distinct cytoplasmic and focally dot-like staining reaction.
Clinical application for IR618IS618. For identification of some epithelial tumors like Merkel cell carcinoma, seminoma and epithelioid hemangioendothelioma.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
FLEX Monoclonal Mouse Anti-Human Cytokeratin 18, Clone DC 10, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels epithelial cells in those epithelia expressing cytokeratin 18, and hence the antibody is a useful tool for the differentiation and identification of epithelial tumors (1) and epithelioid hemangioendotheliomas (2). Antibodies to cytokeratin 18 may also be useful for the identification of seminomas (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
In immunocytochemistry on human cultured cell lines, the antibody labels tumor cells of epithelial origin, whereas normal fibroblasts and tumor cells of non-epithelial origin such as glioma, melanoma and osteosarcoma, are not labeled (7).
Clone: DC 10 (7). Isotype: IgG1, kappa.
- Lane EB, Alexander CM. Use of keratin antibodies in tumor diagnosis [review]. Semin Cancer Biol 1990; 1:165-79.
- Miettinen M, Fetsch JF. Distribution of keratins in normal endothelial cells and a spectrum of vascular tumors: Implications in tumor diagnosis. Hum Pathol 2000; 31:1062-7.
- Fogel M, Lifschitz-Mercer B, Moll R, Kushnir I, Jacob N, Waldherr R, et al. Heterogeneity of intermediate filament expression in human testicular seminomas. Differentiation 1990;45:242-9.
- Lauerová L, Kovarik J, Bártek J, Rejthar A, Vojteek B. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 1988; 7:495-504.
| Product | Size |  | IR618 Clone DC 10
| 60 tests, 12 mL Product No. IR61861 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Liver | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |