Netherlands
FISH Probes
Split Signal Fluorescence In Situ Hybridization
The split signal FISH technique uses two differently labeled DNA-probes flanking each side of a breakpoint region. Consequently, two co-localized yellow (combined green/red) signals will be visible in normal cells, however, a chromosome translocation event will split the co-localized signal, resulting in a separate green and a separate red signal together with a yellow signal from the unaffected chromosome.
MYC gene translocation, Courtesy of Euro-FISH.
Sub-Deletion Signal Fluorescence In Situ Hybridization
The sub-deletion signal FISH technique uses two differently labeled DNA-probes. One probe binds inside the deletion area while the other probe is located outside the deletion area. Consequently, two co-localized yellow (combined green/red) signals will be visible in normal cells, however, a deletion event results in the loss of one red signal from one co-localized signal, thus creating a separate green signal together with a yellow signal from the unaffected chromosome.
T-ALL with SIL-TAL1 gene fusion caused by sub-deletion.
FISH DNA/PNA Probe Mix
Dako's FISH DNA/PNA Probe Mix uses two differently labeled probes in each probe mix; a Texas Red-labeled DNA probe covering the full target gene region, and a mixture of fluorescein-labeled PNA probes directed towards the centromeric region. The combination of a gene-specific probe and probes directed towards the centromere region of the same chromosome allows for detection of the copy number of the specific gene using the centromere as a reference.
The split signal FISH technique uses two differently labeled DNA-probes flanking each side of a breakpoint region. Consequently, two co-localized yellow (combined green/red) signals will be visible in normal cells, however, a chromosome translocation event will split the co-localized signal, resulting in a separate green and a separate red signal together with a yellow signal from the unaffected chromosome.
MYC gene translocation, Courtesy of Euro-FISH.
Sub-Deletion Signal Fluorescence In Situ Hybridization
The sub-deletion signal FISH technique uses two differently labeled DNA-probes. One probe binds inside the deletion area while the other probe is located outside the deletion area. Consequently, two co-localized yellow (combined green/red) signals will be visible in normal cells, however, a deletion event results in the loss of one red signal from one co-localized signal, thus creating a separate green signal together with a yellow signal from the unaffected chromosome.
T-ALL with SIL-TAL1 gene fusion caused by sub-deletion.
FISH DNA/PNA Probe Mix
Dako's FISH DNA/PNA Probe Mix uses two differently labeled probes in each probe mix; a Texas Red-labeled DNA probe covering the full target gene region, and a mixture of fluorescein-labeled PNA probes directed towards the centromeric region. The combination of a gene-specific probe and probes directed towards the centromere region of the same chromosome allows for detection of the copy number of the specific gene using the centromere as a reference.