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Monoclonal Mouse Anti-Human
CD246, ALK Protein
Clone ALK1
Recognizes a formalin-resistant epitope in both the 80 kDa NPM-ALK chimeric and the 200 kDa normal human ALK proteins (1).
Normal ALK protein expression is restricted to the central nervous system. The hybrid gene, NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase (2). This translocation is found in a proportion of cases of anaplastic large cell lymphoma (ALCL).
References
- Pulford K, Lamant L, Morris SW, Butler LH, Wood KM, Stroud D, et al. Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1. Blood 1997;89:1394-404.
- Morris SW, Kirstein MN, Valentine MB, Dittmer KG, Shapiro DN, Saltman DL, et al. Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin's lymphoma (published erratum appears in Science 1995;267:316-7). Science 1994;263:1281-4.
Anaplastic large cell lymphoma (FFPE) stained with FLEX Anti-CD246, ALK Protein, Code IR641/IS641.
Anaplastic large cell lymphoma (t(2;5) translocation). Scattered neoplastic cells show a strong nuclear and cytoplasmic staining reaction.
Clinical application for IR641/IS641. For identification of ALK-positive anaplastic large cell lymphoma.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human CD246, ALK protein, Clone AKL1, Ready-to-Use (Dako Autostainer/ Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels normal human ALK protein and the NPM-ALK chimeric protein, and is useful for the identification of the subgroup of anaplastic large cell lymphomas (ALCL) that are ALK positive (1, 2).The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human CD246, ALK protein, Clone AKL1, Ready-to-Use (Dako Autostainer/ Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels normal human ALK protein and the NPM-ALK chimeric protein, and is useful for the identification of the subgroup of anaplastic large cell lymphomas (ALCL) that are ALK positive (1, 2).The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
DHFR-ALK recombinant protein comprising mouse dihydrofolate reductase (DHFR) and amino acids 1359-1460 of the full length human ALK protein, corresponding to amino acids 419-520 of the chimeric NPM-ALK protein (1).
Specificity
Anti-Human CD246, clone ALK1, was clustered as anti-CD246 at the 7th International Workshop and Conference on Human Leucocyte Differentiation Antigens (3).
In Western blotting of lysate of the t(2;5)+ SU-DHL-1 cell line, the antibody labels a band of 80 kDa corresponding to NPM-ALK protein. The antibody also recognizes a band of 200 kDa corresponding to the ALK protein in the human rhabdomyosarcoma cell line Rh30 that expresses full length ALK protein, and in the 293T cell line transfected with cDNA encoding full length ALK protein. No reactivity was observed when the 293T cell line was transfected with the vector alone (1).
In Western blotting of lysate of the t(2;5)+ SU-DHL-1 cell line, the antibody labels a band of 80 kDa corresponding to NPM-ALK protein. The antibody also recognizes a band of 200 kDa corresponding to the ALK protein in the human rhabdomyosarcoma cell line Rh30 that expresses full length ALK protein, and in the 293T cell line transfected with cDNA encoding full length ALK protein. No reactivity was observed when the 293T cell line was transfected with the vector alone (1).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: ALK1 (1). Isotype: IgG3, kappa.
Clone: ALK1 (1). Isotype: IgG3, kappa.
References
- Pulford K, Lamant L, Morris SW, Butler LH, Wood KM, Stroud D, et al. Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1. Blood 1997;89:1394-404.
- Gascoyne RD, Aoun P, WU D, Chhanabhai M, Skinnider BF, Greiner TC, et al. Prognostic significance of anaplastic lymphoma kinase (ALK) protein expression in adults with anaplastic large cell lymphoma. Blood 1999;93:3913-21.
- Pulford K, Mason DY. TC5. CD246 (ALK) cluster report. In: Mason D, André P, Bensussan A, Buckley C, Civin C, Clark E, et al., editors. Leucocyte typing VII. White cell differentiation antigens. Proceedings of the 7th International Workshop and Conference; 2000 Jun 19-23; Harrogate, United Kingdom. New York: Oxford University Press Inc.; 2002. p. 694-6.
| Product | Size |  | IS641 Clone ALK1
| 30 tests, 6 mL Product No. IS64130 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, Low pH (Code K8005) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800521 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Anaplastic large cell lymphoma with t(2;5) translocation | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX+ (Code K8012) Use Low pH Target Retrieval Solution | 400-600 tests Product No. K801221 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | Silanized Slides (Code S3003) Recommended for greater adherence Alternative is FLEX IHC Microscope Slides (Code K8020) | 100 slides Product No. S300330 |