Norway
LAST PRODUCTS VIEWED
RELATED PRODUCT TAGS
Monoclonal Rabbit Anti-Human
AMACR
Clone 13H4
Recognizes a 382-amino-acid protein, alpha-methylacyl-CoA racemase (AMACR), that was identified by cDNA library subtraction in conjunction with high throughput microarray screening of prostate adenocarcinomas.
AMACR, also known as P504S, is an enzyme that is involved in bile acid biosynthesis and β-oxidation of branched-chain fatty acids. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma (1). In glandular epithelial cells of normal and benign hyperplastic prostates AMACR is present at low or undetectable levels.
- Luo J, Zha S, Gage WR, Dunn TA, Hicks JL, Bennett CJ, et al. Alpha-methylacyl-CoA racemase: a new molecular marker for prostate cancer. Cancer Res 2002;62:220-6.
Prostate adenocarcinoma (FFPE) stained with FLEX Anti-AMACR, Code IR060/IS060.
Prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. A spotty granular cytoplasmic staining is also seen in a few cells of the benign glands.
Clinical application for IR060/IS060. For identification of prostate adenocarcinoma. The majority of the prostatic adenocarcinoma cells should show a granular cytoplasmic staining reaction.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
FLEX Monoclonal Rabbit Anti-Human AMACR, Clone 13H4, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of prostate adenocarcinomas (1-9). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Clone: 13H4 (1-3).
- Xu J, Stolk JA, Zhang X, Silva SJ, Houghton RL, Matsumura M, Vedvick TS, Leslie KB, Badaro R, Reed SG. Identification of differentially expressed genes in human prostate cancer using subtraction and microarray. Canc Res 2000; 60:1677
- Jiang Z, Fanger GR, Woda BA, Banner BF, Algate P, Dresser K, Xu J, Chu PG. Expression of a-methylacyl-CoA racemase (P504S) in various malignant neoplasms and normal tissues: A study of 761 cases. Human Pathology. 2003; 34(8):792
- Jiang Z, Woda BA, Rock KL, Xu Y, Savas L, Khan A, Pihan G, Cai F, Babcook JS, Rathanaswami P, Reed SG, Xu J, Fanger GR. P504S: a new molecular marker for the detection of prostate carcinoma. Am J Surg Pathol. 2001; 25(11):1397
- Jiang Z, Wu C, Woda BA, Dresser K, Xu J, Fanger GR, Yang XJ. P504S/a-methylacyl-CoA racemase: a useful marker for diagnosis of small foci of prostatic carcinoma on needle biopsy. Am J Surg Path. 2002; 26(9):1169
- Yang XJ, Wu C, Woda BA, Dresser K, Tretiakova M, Fanger GR, Jiang Z. Expression of a-methylacyl-CoA racemase (P504S) in atypical adenomatous hyperplasia of the prostate. Am J Surg Path. 2002; 26(7):921
- Zhou M, Jiang Z, Epstein JI. Expression and diagnostic utility of alpha-methylacyl-CoA-racemase (P504S) in foamy gland and pseudohyperplastic prostate cancer. Am J Surg Path. 2003; 27(6):772
- Beach R, Gown AM, de Peralta-Venturina MN, Folpe AL, Yaziji H, Salles PG, Grignon DJ, Fanger GR, Amin MG. P504S immunohistochemical detection in 405 prostatic specimens including 376 18-gauge needle biopsies. Am J Surg Pathol. 2002; 26(12):158
- Wu C-L, Yang XJ, Tretiakova M, Patton KT, Halpern EF, Woda BA, Young RH, Jiang Z. Analysis of a-methylacyl-coA racemase (P504S) expression in high-grade prostatic intraepithelial neoplasia. Hum Pathol 2004;35(8):1008-13
- Jiang Z, Woda B, Wu C-L, Yang X. Discovery and clinical application of a novel prostate cancer marker. Am J Clin Pathol 2004;122:275-89
Check Your Price & Order Online
| Product | Size |  | IR060 Clone 13H4
| 60 tests, 12 mL Product No. IR06061-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IR600) Positive tissue: Epithelial cells of the prostate glands | 120 tests, 24 mL Product No. IR60066-2 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021-2 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |
Synonym(s) for Antigen
a-methylacyl-CoA, alpha-methylacyl-coenzyme A racemase, P504S