Primary Antibodies
Products originating from the same primary antibody have been grouped together. A detailed product description, a guideline for dilution, and a recommended staining procedure are given in the package insert.
Antibody Applications
Suitable tissue fixation and, when necessary, antigen retrieval method are listed for each antibody.
Melanoma (FFPE) stained with FLEX Anti-Melan-A, Code IR633/IS633.
Antibody Applications
Suitable tissue fixation and, when necessary, antigen retrieval method are listed for each antibody.
- Frozen: The antibody is suitable for labeling sections of frozen tissue fixed in acetone for 10 minutes. It also works well on cell smears fixed in a 19:19:1 mixture of acetone:methanol:36% fomalin for 90 seconds, or in acetone for 10 minutes.
- Formalin: The antibody is suitable for labeling formalin-fixed, paraffin-embedded (FFPE) tissue sections. The duration of the formalin fixation should generally be 24-48 hours.
- Enzyme: Optimal staining results require adequate treatment of deparaffinized, formalin-fixed tissue sections with a proteolytic enzyme before incubation with the antibody.
- HIER: Optimal staining results require heat-induced epitope retrieval (HIER) of deparaffinized, formalin-fixed tissue sections in a suitable buffer, e.g. in a microwave oven, a pressure cooker or a water bath, before incubation with the antibody.
- Enzyme/HIER: Optimal staining results on deparaffinized, formalin-fixed tissue sections require either treatment with a proteolytic enzyme or heating in a suitable buffer before incubation with the antibody.
- Enzyme + HIER: Optimal staining results on deparaffinized, formalin-fixed tissue sections require treatment with a proteolytic enzyme as well as heating in a suitable buffer before incubation with the antibody.
Melanoma (FFPE) stained with FLEX Anti-Melan-A, Code IR633/IS633.