Sweden
FAST AND NON-TOXIC ASSAY WITH ACCURATE RESULTS
- 1 hour hybridization, 3-and-a-half hours to results
- Non-toxic solution means no need for fume hoods
- Crisp and clear dual color signals make it faster and easier to identify gene status
- An increased nuclear background staining helps visualize tissue morphology
- No need for blocking or removal of repeat sequences
- Simple and straightforward protocol
- Optional batch pepsin treatment for high throughput and increased robustness
98.7% CONCORDANCE TO STANDARD FISH*
Specificity |
No cross-hybridization to other chromosome targets or interference with closely related substances |
Sensitivity |
HER2/CEN-17 ratio between 0.97-1.08 in normal human breast epithelium |
Reproducibility |
Average coefficient of variation (CV) for observer-to-observer reproducibility of 3.2% for non-amplified specimens and 2.8% for amplified specimens. Lot-to-lot reproducibility of 5% for non-amplified specimens and 7.8% for amplified specimens |
Robustness |
Tested by varying type, time and temperature of the different steps in the assay. Robustness studies showed overall agreement with reference stain of 100%. |
*Dako HER2 IQFISH pharmDx™ (Code K5731) has been compared with Dako HER2 FISH pharmDx™ Kit (Code K5331) in a comparative study. Cross tabulation of HER2 status obtained by the two assays gave an overall agreement of 98.7%. For more details, please refer to the current version of the package insert provided with the HER2 IQFISH pharmDx™ and TOP2A IQFISH pharmDx™ kits.
IQFISH Literature
IQFISH Introducing Instant Quality
(Literature Order No. 29041)