Primary Single-Color Reagents
These primary antibodies are conjugated with a single fluorochrome for use in flow cytometry. After conjugation, unreacted fluorochromes are completely removed by gel filtration. Below is a list of the excitation and emission wavelength of the different flurochromes as well as the approximately molar flurochrome/antibody ration for each fluorochrome.
Allophycocyanin (APC) Conjugates.
The molar APC/antibody ratio is approximately 1. APC conjugates can be excited at 633 nm (HeNe laser) or 635 nm (red diode laser), and emit light at 660 nm.
Cascade Yellow (CY) Conjugates
The molar CY/antibody ratio is approximately 6. CY conjugates can be excited at 406 nm (violet diode laser) and emit light at 541 nm..
Fluorescein (FITC) Conjugates
The molar FITC/antibody ratio is approximately 4. FITC conjugates can be excited at 488 nm (blue) and emit light at 530 nm.
Pacific Blue (PB) Conjugates
The molar PB/antibody ratio is approximately 6. PB conjugates can be excited at 406 nm (violet diode laser) and emit light at 456 nm..
Peridinin Chlorophyll Protein (PerCP) Conjugates
PerCP conjugates can be excited at 488 nm (blue laser) and emit light at 676 nm..
Phycoerythrin (RPE) Conjugates
The molar RPE/antibody ratio is approximately 1. RPE conjugates can be excited at 488 nm (blue) and emit light at 570 nm..
Phycoerythrin-Cy5 (RPE-Cy5) Conjugates
The excitation energy, absorbed at 488 nm by RPE, is transferred to Cy5, which emits light at 670 nm. The molar RPE-Cy5/antibody ratio of the conjugate is approximately 1.
Allophycocyanin (APC) Conjugates.
The molar APC/antibody ratio is approximately 1. APC conjugates can be excited at 633 nm (HeNe laser) or 635 nm (red diode laser), and emit light at 660 nm.
Cascade Yellow (CY) Conjugates
The molar CY/antibody ratio is approximately 6. CY conjugates can be excited at 406 nm (violet diode laser) and emit light at 541 nm..
Fluorescein (FITC) Conjugates
The molar FITC/antibody ratio is approximately 4. FITC conjugates can be excited at 488 nm (blue) and emit light at 530 nm.
Pacific Blue (PB) Conjugates
The molar PB/antibody ratio is approximately 6. PB conjugates can be excited at 406 nm (violet diode laser) and emit light at 456 nm..
Peridinin Chlorophyll Protein (PerCP) Conjugates
PerCP conjugates can be excited at 488 nm (blue laser) and emit light at 676 nm..
Phycoerythrin (RPE) Conjugates
The molar RPE/antibody ratio is approximately 1. RPE conjugates can be excited at 488 nm (blue) and emit light at 570 nm..
Phycoerythrin-Cy5 (RPE-Cy5) Conjugates
The excitation energy, absorbed at 488 nm by RPE, is transferred to Cy5, which emits light at 670 nm. The molar RPE-Cy5/antibody ratio of the conjugate is approximately 1.
Products
- B Cell
- BCL2 Oncoprotein
- Bromodeoxyuridine
- CD1a
- CD2
- CD3
- CD4
- CD5
- CD7
- CD7
- CD8
- CD10
- CD11b, C3bi Receptor
- CD11c, Protein 150,95
- CD13
- CD14
- CD15
- CD16, Fc Gamma Receptor III
- CD19
- CD20
- CD22
- CD23
- CD24
- CD25, Interleukin-2 Receptor
- CD27
- CD28
- CD30
- CD33
- CD34 Class III
- CD38
- CD41, Platelet Glycoprotein IIb
- CD42b, Platelet Glycoprotein Ib
- CD43
- CD45, Leucocyte Common Antigen
- CD45, Leucocyte Common Antigen
- CD45R0
- CD45RA
- CD54, ICAM-1
- CD56
- CD56
- CD57
- CD61, Platelet Glycoprotein IIIa
- CD64, Fc Gamma Receptor I
- CD66abce
- CD68
- CD69
- CD71, Transferrin Receptor
- CD79αcy
- CD79β
- CD86
- CD90
- CD95, Fas
- CD103, Mucosa Lymphocyte Antigen (MLA)
- CD117, c-kit
- CD138
- CD235a, Glycophorin A
- Epithelial Antigen
- HLA-ABC Antigen
- HLA-DP, DQ, DR Antigen
- HLA-DR Antigen
- IgA, Specific for Alpha-Chains
- IgD, Specific for Delta-Chains
- IgG, Specific for Gamma-Chains
- IgM, Specific for Mu-Chains
- Kappa Light Chains
- Ki-67 Antigen
- Ki-67 Antigen
- Lactoferrin
- Lambda Light Chains
- Lysozyme EC 3.2.1.17
- Myeloperoxidase
- Plasma Cell
- Terminal Deoxynucleotidyl Transferase

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