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Monoclonal Mouse Anti-Human
Carcinoembryonic Antigen (CEA)
Clone II-7
Monoclonal antibodies to CEA have been classified into five essentially non-interacting epitope groups, designated Gold 1 to 5. Dako Anti-CEA, clone II-7, belongs to epitope group Gold 1 and shows a high degree of CEA specificity (1).
CEA is a clinically important marker for adenocarcinomas, notably in the gastrointestinal tract, including colonic and pancreatic carcinomas. The characteristic CEA expression of secretory meningiomas is labeled by the antibody (2). Antibodies to CEA may also be useful for the identification of medullary carcinoma of the thyroid (3).
References
- Nap M, Hammarström ML, Börmer O, Hammarström S, Wagener C, Handt S, et al. Specificity and affinity of monoclonal antibodies against carcinoembryonic antigen. Cancer Res 1992;52:2329-39.
- Probst-Cousin S, Villagran-Lillo R, Lahl R, Bergmann M, Schmid KW, Gullotta F. Secretory meningioma. Clinical, histologic, and immunohistochemical findings in 31 cases. Cancer 1997;79:2003-15.
- Uribe M, Fenoglio-Preisler CM, Grimes M, Feind C. Medullary carcinoma of the thyroid gland. Am J Surg Pathol 1985;9:577-94.
Colon adenocarcinoma (FFPE) stained with FLEX Anti-CEA, Code IR622/IS622.
Colon adenocarcinoma. The majority of the neoplastic cells show a distinct and predominantly cytoplasmic staining reaction.
Clinical application for IR622/IS622. For identification of carcinoembryonic antigen (CEA)-positive glycocalyx surface of gastrointestinal cells and colon carcinoma.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Carcinoembryonic Antigen (CEA), Clone II-7, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with (Dako Autostainer/ Autostainer Plus instruments. This antibody labels the CEA-positive glycocalyx surface of gastrointestinal cells and is useful for the identification of colon carcinomas (1). Antibodies to CEA may also be useful for the identification of medullary carcinoma of the thyroid (2). In addition, the characteristic CEA expression of secretory meningiomas, is labeled by the antibody (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Carcinoembryonic Antigen (CEA), Clone II-7, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with (Dako Autostainer/ Autostainer Plus instruments. This antibody labels the CEA-positive glycocalyx surface of gastrointestinal cells and is useful for the identification of colon carcinomas (1). Antibodies to CEA may also be useful for the identification of medullary carcinoma of the thyroid (2). In addition, the characteristic CEA expression of secretory meningiomas, is labeled by the antibody (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Purified human CEA from liver metastasis of lung adenocarcinoma (4).
Specificity
In ELISA, the antibody reacts with CEA preparations from both colo-rectal carcinomas, lung andenocarcinomas and the colon carcinoma cell line LS 174T, whereas the antibody does not cross-react with isolated CEA-related antigens, e.g. NCA-55, NCA-95, NCA-160 and BGP I (4). Based on ELISA, using reduced, carboxymethylated and Smith degraded CEA, the antibody is predicted to react with a conformation dependent epitope, in which the carbohydrate structures of the CEA glycoprotein are involved (4).
Using solid-phase competitive immunoassays, monoclonal antibodies to CEA have been classified into five essentially non-interacting epitope groups called GOLD 1-5. Monoclonal antibodies with a high degree of CEA specificity generally belong to epitope groups GOLD 1 or 3. Monoclonal Mouse Anti-Human Carcinoembryonic Antigen (CEA), Clone II-7, belongs to epitope group GOLD 1 (8).
Using solid-phase competitive immunoassays, monoclonal antibodies to CEA have been classified into five essentially non-interacting epitope groups called GOLD 1-5. Monoclonal antibodies with a high degree of CEA specificity generally belong to epitope groups GOLD 1 or 3. Monoclonal Mouse Anti-Human Carcinoembryonic Antigen (CEA), Clone II-7, belongs to epitope group GOLD 1 (8).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: II-7 (3). Isotype: IgG1, kappa.
Clone: II-7 (3). Isotype: IgG1, kappa.
References
- Zoubir F, Zeromski J, Sikora J, Szmeja J, Hedin A, Hammarström S. Tumor specificity of monoclonal antibodies to carcinoembryonic antigen. Tumor Biol 1990;11:5-19.
- Probst-Cousin S, Villagran-Lillo R, Lahl R, Bergmann M, Schmid KW, Gullotta F. Secretory meningioma. Clinical, histologic, and immunohistochemical findings in 31 cases. Cancer 1997; 79:2003-15.
- Uribe M, Fenoglio-Preisler CM, Grimes M, Feind C. Medullary carcinoma of the thyroid gland. Am J Surg Pathol 1985;9:577-94.
- Hedin A, Zoubir F, Lundgren T, Hammarström S. Epitope specificity and cross-reactivity pattern of a large series of monoclonal antibodies to carcinoembryonic antigen. Mol Immunol 1986; 23:1053-61.
- Hammerstrom S, Shively JE, Paxton RJ, Beatty BG, Larsson Å, Ghosh R, et al. Antigenic sites in carcinoembryonic antigen. Cancer Res 1989;49:4852-8.
Check Your Price & Order Online
| Product | Size |  | IS622 Clone II-7
| 30 tests, 6 mL Product No. IS62230-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Colon and tonsil | 60 tests, 12 mL Product No. IS75061-2 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |
Synonym(s) for Antigen
CEA