Monoclonal Mouse Anti-

Thyroid Transcription Factor (TTF-1)

Clone 8G7G3/1

Identifies the 40 kDa TTF-1 band in nuclear extracts or whole cell lysates of TTF-1-positive cell lines of rat, mouse and man.

TTF-1 is selectively expressed in lung and thyroid, and the antibody may aid in the classification of tumors of the lung and thyroid.

References
  1. Holzinger A, Dingle S, Bejarano PA, Miller MA, Weaver TE, DiLauro R, et al. Monoclonal antibody to thyroid transcription factor 1: production, characterization, and usefulness in tumor diagnosis. Hybridoma 1996;15:49-53.

Lung smal cell carcinoma (FFPE) stained with Anti-TTF-1, Code IR056/IS056.

+

Lung small cell carcinoma. The neoplastic cells show a moderate to strong nuclear staining reaction.

Clinical application for IR056/IS056. For differential diagnosis of primary vs. metastatic tumors in the lung and thyroid. Mesothelioma is negative with this marker.

Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.

  • Optimized staining performance of both high- and low-expression structures
  • Crisp and clear staining with no background
  • Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy

Intended Use
For in vitro diagnostic use.

FLEX Monoclonal Mouse Anti-Thyroid Transcription Factor, Clone 8G7G3/1, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of tumors from lung and thyroid. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Recombinant rat TTF-1 (1)
Specificity
Monoclonal anti-rat TTF-1 clone 8G7G3/1 (anti-TTF-1) has also been shown to react with rat, mouse and human TTF-1. In Western blotting, anti-TTF-1 specifically identified a 40 kDa band in immunoblots of nuclear extracts or whole cell lysates from the TTF-1 positive cell lines MLE 15 (mouse lung epithelial), H441-4 (human lung adenocarcinoma), H 345 (human small cell carcinoma) and rat type II pneumocyte cells. Cell lines lacking TTF-1 mRNA such as HeLa and 3T3 were unreactive. Anti-TTF-1 was also found to function in ELISA assays using recombinant TTF-1 (1).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.

Clone: 8G7G3/1 (1). Isotype: IgG1, kappa
References
  1. Holzinger A, Dingle S, Bejarano PA, Miller M-A, Weaver TE, Di Lauro R, Whitsett JA. Monoclonal antibody to thyroid transcription factor-1: Production, characterization and usefulness in tumor diagnosis. Hybridoma 1996;15:49-53.
ProductSize
This product is CE-marked according to the EU IVD Directive 98/79/ECIS056 Clone 8G7G3/1
Specification Sheet
Safety Data Sheet:
Non-hazard classification statement
30 tests, 6 mL
Product No. IS05630-2

RECOMMENDED COMPANION PRODUCTS

StepProductSize
Pre-treatmentEnVision™ FLEX Target Retrieval Solution, High pH (Code K8004)
3-in-1 procedure using PT Link
3 x 30 mL
Product No. K800421
Antibody Dilution
Ready-to-use / Prediluted
 
Negative ControlFLEX Negative Control, Mouse (Code IS750)
Positive tissue: Thyroid follicular epithelial cells
60 tests, 12 mL
Product No. IS75061-2
Visualization SystemEnVision™ FLEX+ (Code K8012)
Use High pH Target Retrieval Solution
400-600 tests
Product No. K801221
CounterstainHematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018)
Non-aqueous, permanent mounting medium is required
10 x 13 mL, 400-600 tests
Product No. K801821
Glass SlidesSilanized Slides (Code S3003)
Recommended for greater adherence
Alternative is FLEX IHC Microscope Slides (Code K8020)
100 slides
Product No. S300330-2

Synonym(s) for Antigen

TTF-1, TTF1

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