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Monoclonal Mouse Anti-Human
Alpha-1-Antitrypsin
Alpha-1-antitrypsin (A1AT) is a 51 kDa glycoprotein mainly synthesized in the liver.
It is an acute phase protein and functions as a major inhibitor of serine proteases. A1AT is present in normal liver cells, histiocytes and monocytes, and in a large variety of tumors of both epithelial and mesenchymal differentiation.
A1AT deficient liver (FFPE) stained with FLEX Anti-Alpha-1-Antitrypsin, Code IR505/IS505.
A1AT deficient liver. The hepatocytes with alpha-1-antitrypsin deficiency show a strong granular cytoplasmic staining reaction (seen in a standard HE stained slide as an accumulation of eosinophilic lysosomes), while normal hepatocytes show a weak staining reaction.
Clinical application for IR505/IS505. Primarily for identification of alpha-1-antitrypsin (A1AT) accumulation in the hepatocytes of A1AT deficient liver.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
FLEX Polyclonal Rabbit Anti-Human Alpha-1-Antitrypsin, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of alpha-1-antitrypsin, e.g. in hepatocytes in alpha-1-antitrypsin deficiency (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Crossed immunoelectrophoresis: Only the alpha-1-antitrypsin precipitation arch appears when using 12.5 µL Dako Code A0012 per cm2 gel area against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
- Clausen PP. Immunohistochemical demonstration of alpha-1-antitrypsin in liver tissue. A methodological investigation. Acta path microbiol scand Sect A 1980;88:299-306.
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| Product | Size |  | IS505
| 30 tests, 6 mL Product No. IS50530-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IS600) Positive tissue: Tonsil and liver | 60 tests, 12 mL Product No. IS60061-2 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |
Synonym(s) for Antigen
AAT, A1AT