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Polyclonal Rabbit Anti-Human
IgA
The antigen used for immunization is serum IgA. The very high specificity and the good performance of the antibody have been ascertained in immunohistochemistry as well as in indirect ELISA and immunoblotting.
Additionally, the specificity has been tested by crossed immunoelectrophoresis using 12.5 microlitre antibody per square cm gel area against 2 microlitre human plasma.
The F(ab')2 fragment antibody is particularly useful for labeling unfixed blood cells containing active Fc receptors, and for other applications where the Fc part of the antibody molecule could disturb.
Tonsil (FFPE) stained with FLEX Anti-IgA, Code IR510/IS510.
Tonsil. Plasma cells and immunoblasts show a moderate to strong cytoplasmic staining reaction.
Clinical application for IR510/IS510. For identification of plasma cells and related lymphoid cells containing IgA, and for the classification of B-cell neoplasias.
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- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
FLEX Polyclonal Rabbit Anti-Human IgA, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of plasma cells and related lymphoid cells containing IgA, and it is a useful tool for the classification of patients with B-cell neoplasias (1-3). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of plasma cells (1, 4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
The specificity of the antibody has been ascertained as follows:
Crossed immunoelectrophoresis: Only the IgA immunoprecipitation arch appears when using 12.5 µL concentrated antibody per square cm gel area against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
ELISA: No significant reaction is seen in indirect ELISA using human IgG and IgM as coating antigens. In double antibody sandwich ELISA no significant reaction is observed with human plasma stripped of IgA.
- Taylor CR, Burns J. The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labeled antibody. J Clin Pathol 1974;27:14-20.
- Taylor CR, Mason DY. The immunohistological detection of intracellular immunoglobulin in formalin-paraffin sections from multiple myeloma and related conditions using the immunoperoxidase technique. Clin exp Immunol 1974;18:417-29.
- Taylor CR, Russell R, Chandor S. An immunohistologic study of multiple myeloma and related conditions, using an immunoperoxidase method. Am J Clin Pathol 1978;70:612-22.
- Beschorner R, Horny H-P, Petrusch UR, Kaiserling E. Frequent expression of haemopoietic and non-haemopoietic antigens by reactive plasma cells: an immunohistochemical study using formalin-fixed, paraffin-embedded tissue. Histol Histopathol 1999;14:805-12.
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| Product | Size |  | IS510
| 30 tests, 6 mL Product No. IS51030-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IS600) Positive tissue: Tonsil | 60 tests, 12 mL Product No. IS60061-2 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |