Reacts with the wild type and mutant type of the p53 protein.
The antibody is useful for demonstrating accumulation of p53 protein, which occurs in a high frequency in tumors of all cell lineages.
Serous ovarian carcinoma (FFPE) stained with FLEX Anti-p53 Protein, Code IR616/IS616.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains
- a guide to diagnostic accuracy
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human p53 Protein, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels wild-type and mutant-type p53 protein and is useful for the identification of p53 accumulation in human neoplasias (1-3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Recombinant human wild-type p53 protein (1).
In Western blotting of lysate of the A431 human vulval carcinoma cell line, the antibody labels a 53 kDa band, corresponding to the mutant type p53, which is expressed by A431. The epitope recognized by the antibody is located between the N-terminal amino acids 1 and 45 and possibly between amino acids 37 and 45 of the human p53 protein (1).
SDS-PAGE analysis of immunoprecipitates formed between lysate of the BT474 breast cancer cell line and the antibody shows reaction with a 53 kDa protein corresponding to p53 (1).
In immunohistochemistry the antibody labels mutant-type p53 in the A431 cell line and wild-type p53 in the SVK14 cell line (SV40-transformed keratinocyte line) (1).
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: DO-7 (1). Isotype: IgG2b, kappa.
- Vojteek B, Bártek J, Midgley CA, Lane DP. An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53. J Immunol Methods 1992;151:237-44.
- Nieder C, Petersen S, Petersen C, Thames HD. The challenge of p53 as prognostic and predictive factor in Hodgkin's or non-Hodgkin's lymphoma (review). Ann Hematol 2001;80:2-8.
- Ramael M, Lemmens G, Eerdekens C, Buysse C, Deblier I, Jacobs W, et al. Immunoreactivity for p53 protein in malignant mesothelioma and non-neoplastic mesothelium. J Pathol 1992;168:371-5.
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