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Monoclonal Mouse Anti-Human
p53 Protein
Clone DO-7
Reacts with the wild type and mutant type of the p53 protein.
The antibody is useful for demonstrating accumulation of p53 protein, which occurs in a high frequency in tumors of all cell lineages.
Serous ovarian carcinoma (FFPE) stained with FLEX Anti-p53 Protein, Code IR616/IS616.
Serous ovarian carcinoma. Virtually all the neoplastic cells show a moderate to strong nuclear staining reaction.
Clinical application for IR616/IS616. For identification of wild-type and mutant-type p53 protein and accumulation in human neoplasia.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
FLEX Monoclonal Mouse Anti-Human p53 Protein, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels wild-type and mutant-type p53 protein and is useful for the identification of p53 accumulation in human neoplasias (1-3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
SDS-PAGE analysis of immunoprecipitates formed between lysate of the BT474 breast cancer cell line and the antibody shows reaction with a 53 kDa protein corresponding to p53 (1).
In immunohistochemistry the antibody labels mutant-type p53 in the A431 cell line and wild-type p53 in the SVK14 cell line (SV40-transformed keratinocyte line) (1).
Clone: DO-7 (1). Isotype: IgG2b, kappa.
- Vojteek B, Bártek J, Midgley CA, Lane DP. An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53. J Immunol Methods 1992;151:237-44.
- Nieder C, Petersen S, Petersen C, Thames HD. The challenge of p53 as prognostic and predictive factor in Hodgkin's or non-Hodgkin's lymphoma (review). Ann Hematol 2001;80:2-8.
- Ramael M, Lemmens G, Eerdekens C, Buysse C, Deblier I, Jacobs W, et al. Immunoreactivity for p53 protein in malignant mesothelioma and non-neoplastic mesothelium. J Pathol 1992;168:371-5.
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| Product | Size |  | IS616 Clone DO-7
| 30 tests, 6 mL Product No. IS61630-2 |
|---|