For in vitro diagnostic use.

FLEX Monoclonal Mouse Anti-Human CD1a, Clone O10, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the classification of thymomas and malignancies of T-cell precursors and the diagnosis of Langerhans' cell histiocytosis (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Human thymus cells.

Anti-Human CD1a, clone O10, was clustered as anti-CD1a at the 4th International Workshop and Conference on Human Leucocyte Differentiation Antigens (2). Clone O10 has been shown to immunoprecipitate labeled CD1a from thymus cell lysate and from Langerhans' cell-enriched epidermal cell lysate (2). In the latter, the antibody strongly precipitated the native CD1a molecule (49 kDa molecular weight) and β₂-microglobulin (12 kDa molecular weight) but not the trypsin-cleaved CD1a molecule (27 kDa molecular weight) (2). In immunohistochemistry, the antibody has been demonstrated to react with the CD1a cell surface glycoprotein expressed on cortical thymocytes, Langerhans' cells, and interdigitating dendritic (reticulum) cells in frozen and formalin-fixed, paraffin-embedded (FFPE) tissues (1, 2).

Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide. Clone: O10 (1). Isotype: IgG1, kappa.