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Monoclonal Mouse Anti-Human
Actin (Smooth Muscle)
Clone 1A4
This antibody labels smooth muscle cells, myofibroblasts and myoepithelial cells.
It is useful for the identification of leiomyomas, leiomyosarcomas (1), and pleomorphic adenomas (2).
Isotype IgG2a, kappa
- Frozen
- Formalin
- HIER
References
- Rizeq MN, van de Rijn M, Hendrickson MR, Rouse RV. A comparative immunohistochemical study of uterine smooth muscle neoplasms with emphasis on the epithelioid variant. Hum Pathol 1994;25:671-7.
- Brennan PA, Umar T, Zaki GA, Langdon JD, Spedding A, Buckley J, et al. Are myoepithelial cells responsible for the widespread expression of inducible nitric oxide synthase in pleomorphic adenoma? An immunohistochemical study. J Oral Pathol Med 2000;29:279-83.
Leiomyosarcoma (FFPE) stained with FLEX Anti-Actin (Smooth Muscle), Code IR611/IS611.
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Leiomyosarcoma. The majority of the neoplastic cells show a moderate to strong cytoplasmic staining reaction.
Clinical application for IR611/IS611. For identification of smooth muscle cells, myofibroblasts, and myoepithelial cells and their tumors such as leiomyoma and leiomyosarcoma.
Uterine leiomyoma. The majority of the neoplastic cells show a moderate to strong cytoplasmic staining reaction.
Clinical application for IR611/IS611. For identification of smooth muscle cells, myofibroblasts, and myoepithelial cells and their tumors such as leiomyoma and leiomyosarcoma.
Colon. The smooth muscle cells show a moderate to strong cytoplasmic staining reaction. No staining reaction should be seen in the epithelial cells.
Recommended control for IR611/IS611. Appendix/Colon/Breast/Liver.
Liver. The smooth muscle cells lining the sinusoids show a weak to moderate cytoplasmic staining reaction.
Recommended control for IR611/IS611. Appendix/Colon/Breast/Liver.
Colon stained with Anti-Actin (Smooth Muscle).
Recommended control for IR611/IS611. Appendix/Colon/Breast/Liver.
Appendix stained with Anti-Actin (Smooth Muscle).
Recommended control for IR611/IS611. Appendix/Colon/Breast/Liver.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human Smooth Muscle Actin, Clone 1A4, is intended for use in immunohistochemistry. The antibody labels smooth muscle cells, myofibroblasts and myoepithelial cells, and is a useful tool for the identification of leiomyomas, leiomyosarcomas (1, 2), and pleomorphic adenomas (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human Smooth Muscle Actin, Clone 1A4, is intended for use in immunohistochemistry. The antibody labels smooth muscle cells, myofibroblasts and myoepithelial cells, and is a useful tool for the identification of leiomyomas, leiomyosarcomas (1, 2), and pleomorphic adenomas (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
N-terminal synthetic decapeptide of α-smooth muscle actin (4)
Specificity
In Western blotting and SDS-PAGE immunoblotting of the α-smooth muscle isoform of actin, the antibody labels a band corresponding to α-smooth muscle actin (4).
As demonstrated by Western blotting and/or immunohistochemistry, the antibody cross-reacts with the α-smooth muscle actin-equivalent protein in chicken, cow and rat (4).
As demonstrated by Western blotting and/or immunohistochemistry, the antibody cross-reacts with the α-smooth muscle actin-equivalent protein in chicken, cow and rat (4).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: 1A4. The 1A4 clone is identical to the anti-asm-1 described in (4). Isotype: IgG2a, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
Clone: 1A4. The 1A4 clone is identical to the anti-asm-1 described in (4). Isotype: IgG2a, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
References
- Roholl PJM, Elbers HRJ, Prinsen I, Claessens JAJ, van Unnik JAM. Distribution of actin isoforms in sarcomas: an immunohistochemical study. Hum Pathol 1990;21:1269-74.
- Rizeq MN, van de Rijn M, Hendrickson MR, Rouse RV. A comparative immunohistochemical study of uterine smooth muscle neoplasms with emphasis on the epithelioid variant. Hum Pathol 1994;25:671-7.
- Brennan PA, Umar T, Zaki GA, Langdon JD, Spedding A, Buckley J, et al. Are myoepithelial cells responsible for the widespread expression of inducible nitric oxide synthase in pleomorphic adenoma? An immunohistochemical study. J Oral Pathol Med 2000;29:279-83.
- Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D, Gabbian G. A monoclonal antibody against ¿-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol 1986;103:2787-96.
Check Your Price & Order Online
| Product | Size |  | M0851 Culture supernatant
| 0.2 mL Product No. M085129-2 |
|---|---|---|
| 1 mL Product No. M085101-2 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Smooth Muscle Actin, Clone 1A4, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels smooth muscle cells, myofibroblasts and myoepithelial cells, and is useful for the identification of leiomyomas, leiomyosarcomas (1), and pleomorphic adenomas (2). Differential identification is aided by the results from a panel of antibodies. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Smooth Muscle Actin, Clone 1A4, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels smooth muscle cells, myofibroblasts and myoepithelial cells, and is useful for the identification of leiomyomas, leiomyosarcomas (1), and pleomorphic adenomas (2). Differential identification is aided by the results from a panel of antibodies. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
N-terminal synthetic decapeptide of α-smooth muscle actin (4).
Specificity
In Western blotting and SDS-PAGE immunoblotting of the α-smooth muscle isoform of actin, the antibody labels a band corresponding to α-smooth muscle actin (3).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: 1A4. The 1A4 clone is identical to the anti-asm-1 described in (3). Isotype: IgG2a, kappa.
Clone: 1A4. The 1A4 clone is identical to the anti-asm-1 described in (3). Isotype: IgG2a, kappa.
References
- Rizeq MN, van de Rijn M, Hendrickson MR, Rouse RV. A comparative immunohistochemical study of uterine smooth muscle neoplasms with emphasis on the epithelioid variant. Hum Pathol 1994; 25:671-7.
- Brennan PA, Umar T, Zaki GA, Langdon JD, Spedding A, Buckley J, et al. Are myoepithelial cells responsible for the widespread expression of inducible nitric oxide synthase in pleomorphic adenoma? An immunohistochemical study. J Oral Pathol Med 2000;29:279-83.
- Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D, Gabbian G. A monoclonal antibody against α-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol 1986; 103:2787-96.
- Roholl PJM, Elbers HRJ, Prinsen I, Claessens JAJ, van Unnik JAM. Distribution of actin isoforms in sarcomas: an immunohistochemical study. Hum Pathol 1990; 21:1269-74.
Check Your Price & Order Online
| Product | Size | | IR611 RTU, FLEX
| 60 tests, 12 mL Product No. IR61161-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Colon and liver | 120 tests, 24 mL Product No. IR75066-2 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021-2 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Smooth Muscle Actin, Clone 1A4, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels smooth muscle cells, myofibroblasts and myoepithelial cells, and is useful for the identification of leiomyomas, leiomyosarcomas (1), and pleomorphic adenomas (2). Differential identification is aided by the results from a panel of antibodies. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Smooth Muscle Actin, Clone 1A4, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels smooth muscle cells, myofibroblasts and myoepithelial cells, and is useful for the identification of leiomyomas, leiomyosarcomas (1), and pleomorphic adenomas (2). Differential identification is aided by the results from a panel of antibodies. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
N-terminal synthetic decapeptide of α-smooth muscle actin (4).
Specificity
In Western blotting and SDS-PAGE immunoblotting of the α-smooth muscle isoform of actin, the antibody labels a band corresponding to α-smooth muscle actin (3).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: 1A4. The 1A4 clone is identical to the anti-asm-1 described in (3). Isotype: IgG2a, kappa.
Clone: 1A4. The 1A4 clone is identical to the anti-asm-1 described in (3). Isotype: IgG2a, kappa.
References
- Rizeq MN, van de Rijn M, Hendrickson MR, Rouse RV. A comparative immunohistochemical study of uterine smooth muscle neoplasms with emphasis on the epithelioid variant. Hum Pathol 1994; 25:671-7.
- Brennan PA, Umar T, Zaki GA, Langdon JD, Spedding A, Buckley J, et al. Are myoepithelial cells responsible for the widespread expression of inducible nitric oxide synthase in pleomorphic adenoma? An immunohistochemical study. J Oral Pathol Med 2000;29:279-83.
- Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D, Gabbian G. A monoclonal antibody against α-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol 1986; 103:2787-96.
- Roholl PJM, Elbers HRJ, Prinsen I, Claessens JAJ, van Unnik JAM. Distribution of actin isoforms in sarcomas: an immunohistochemical study. Hum Pathol 1990; 21:1269-74.
Check Your Price & Order Online
| Product | Size | | IS611 RTU, FLEX
| 30 tests, 6 mL Product No. IS61130-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Colon and liver | 60 tests, 12 mL Product No. IS75061-2 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021-2 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |
Synonym(s) for Antigen
SMA, Smooth Muscle Alpha