For in vitro diagnostic use.
F0117, FITC-Conjugated Polyclonal Rabbit Anti-Human Albumin is intended for the demonstration of albumin in tissue sections, and may also be used for other immunofluorescence techniques. Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
The antibody reacts with human albumin. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
The specificity has been ascertained as follows:
Crossed immunoelectrophoresis: Only the albumin precipitation arch appears when using unconjugated antibody corresponding to 50 µL F 0117 per square cm gel area against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
The Anti-Human Albumin conjugate, F0117, has been produced from a purified immunoglobulin fraction of rabbit antiserum. F 0117 is provided in liquid form in phosphate buffer containing 15 mmol/L NaN3, pH 7.2.
Protein concentration g/L: See label on vial.
Antibody titre: 200 mg/L (mg of antigen to be added to 1 L of conjugate to reach equivalence point).
F/P ratio: E495 nm/E278 nm = 0.65 ± 0.05 corresponding to a molar FITC/protein ratio of 2.5.