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Monoclonal Mouse Anti-Human
BCL2 Oncoprotein
Clone 124
Reacts with the BCL2 oncoprotein encoded by a gene involved in the t(14;18) chromosomal translocation. The BCL2 oncoprotein plays a central role in apoptosis.
This antibody has found numerous applications in studies of apoptosis, e.g. in hematological malignancies and other malignant diseases (1, 2). The antibody may be useful in the classification of follicular lymphomas and various diffuse lymphoproliferative diseases, and for differential identification of diffuse large B-cell lymphoma versus Burkitt lymphoma/leukemia (3).
Isotype IgG1, kappa
- Frozen
- Formalin
- HIER
References
- Pezzella F, Turley H, Kuzu I, Tungekar MF, Dunnill MS, Pierce CB, et al. Bcl-2 protein in non-small-cell lung carcinoma. N Engl J Med 1993;329:690-4.
- Campos L, Rouault JP, Sabido O, Oriol P, Roubi N, Vasselon C, et al. High expression of bcl-2 protein in acute myeloid leukemia cells is associated with poor response to chemotherapy. Blood 1993;81:3091-6.
- Haralambieva E, Boerma E-J, van Imhoff GW, Rosati S, Schuuring E, Müller-Hermelink HK, et al. Clinical, immunophenotypic, and genetic analysis of adult lymphomas with morphologic features of Burkitt lymphoma. Am J Surg Pathol 2005;29:1086-94.
Follicular lymphoma (FFPE) stained with FLEX Anti-BCL2 Oncoprotein, Code IR614/IS614.
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Follicular lymphoma. The majority of the neoplastic cells show a distinct cytoplasmic staining reaction.
Clinical application for IR614/IS614.. Primarily for identification of follicular lymphoma. Various B- and T-cell lymphoproliferative diseases and some diffuse large B-cell lymphomas are BCL2 positive. Burkitt lymphoma/leukemia is generally negative for BCL2.
Burkitt lymphoma/leukemia. The neoplastic cells are negative for BCL2 and only benign T cells are stained.
Clinical application for IR614/IS614. Primarily for identification of follicular lymphoma. Various B- and T-cell lymphoproliferative diseases and some diffuse large B-cell lymphomas are BCL2 positive. Burkitt lymphoma/leukemia is generally negative for BCL2.
Tonsil. The scattered T cells in the germinal centers and the mantle zone B cells show a moderate to strong cytoplasmic staining reaction. The germinal center B cells are negative.
Recommended control for IR614/IS614. Tonsil.
Tonsil. The basal epithelial cells show a weak to moderate staining reaction.
Recommended control for IR614/IS614. Tonsil.
Follicular lymphoma stained with Anti-BCL2 Oncoprotein.
Clinical application for IR614/IS614. Primarily for identification of follicular lymphoma. Various B- and T-cell lymphoproliferative diseases and some diffuse large B-cell lymphomas are BCL2 positive. Burkitt lymphoma/leukemia is generally negative for BCL2.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human BCL2 Oncoprotein, Clone 124, is intended for use in immunohistochemistry. The antibody labels cells expressing BCL2 oncoprotein. Positive results aid in the classification of follicular lymphomas and various diffuse lymphoproliferative diseases. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human BCL2 Oncoprotein, Clone 124, is intended for use in immunohistochemistry. The antibody labels cells expressing BCL2 oncoprotein. Positive results aid in the classification of follicular lymphomas and various diffuse lymphoproliferative diseases. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Synthetic peptide comprising amino acids 41-54 of human BCL2 oncoprotein (1).
Specificity
In Western blotting of extracts of normal human spleen (1, 5), t(14;18)-positive follicular lymphoma (1), and myloid leukaemic cell lines (5) the antibody labels solely a band of 26 kDa, corresponding to BCL2 oncoprotein under both nonreducing (1) and reducing conditions (1, 5).
The antibody labels the myeloid leukaemic cell lines, HL-60 (promyelocytic), KG1 (myeloblastic), GM-1 (monoblastic) and K562 (erythromyeloid) (5).
The antibody labels the myeloid leukaemic cell lines, HL-60 (promyelocytic), KG1 (myeloblastic), GM-1 (monoblastic) and K562 (erythromyeloid) (5).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: 124 (1). Isotype: IgG1, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is adjusted to ascertain comparable lot-to-lot immunohistochemical staining performance with a reference lot.
Clone: 124 (1). Isotype: IgG1, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is adjusted to ascertain comparable lot-to-lot immunohistochemical staining performance with a reference lot.
References
- Pezzella F, Tse AGD, Cordell JL, Pulford KAF, Gatter KC, Mason DY. Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation. Am J Pathol 1990;137:225-32.
- Delia D, Aiello A, Soligo D, Fontanella E, Melani C, Pezzella F, et al. bcl-2 proto-oncogene expression in normal and neoplastic human myeloid cells. Blood 1992;79:1291-8.
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| Product | Size |  | M0887 Culture supernatant
| 0.2 mL Product No. M088729-2 |
|---|---|---|
| 1 mL Product No. M088701-2 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human BCL2 Oncoprotein, Clone 124, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels cells expressing BCL2 oncoprotein. Positive results aid in the classification of follicular lymphomas and various diffuse lymphoproliferative diseases (1). The antibody is a useful tool in the differential diagnosis of Burkitt lymphoma/leukemia versus diffuse large B-cell lymphoma (2).The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human BCL2 Oncoprotein, Clone 124, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels cells expressing BCL2 oncoprotein. Positive results aid in the classification of follicular lymphomas and various diffuse lymphoproliferative diseases (1). The antibody is a useful tool in the differential diagnosis of Burkitt lymphoma/leukemia versus diffuse large B-cell lymphoma (2).The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Synthetic peptide comprising amino acids 41-54 of human BCL2 oncoprotein (1).
Specificity
In Western blotting of extracts of normal human spleen (1, 6), t(14;18)-positive follicular lymphoma (1), and myloid leukemia cell lines (6) the antibody labels solely a band of 26 kDa, corresponding to BCL2 oncoprotein under both nonreducing (1) and reducing conditions (1, 6). The antibody labels the myeloid leukemia cell lines, HL-60 (promyelocytic), KG1 (myeloblastic), GM-1 (monoblastic) and K562 (erythromyeloid) (6).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: 124 (1). Isotype: IgG1, kappa.
Clone: 124 (1). Isotype: IgG1, kappa.
References
- Pezzella F, Tse AGD, Cordell JL, Pulford KAF, Gatter KC, Mason DY. Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation. Am J Pathol 1990; 137:225-32.
- Haralambieva E, Boerma E-J, van Imhoff GW, Rosati S, Schuuring E, Müller-Hermelink HK, et al. Clinical, immunophenotypic, and genetic analysis of adult lymphomas with morphologic features of Burkitt lymphoma. Am J Surg Pathol 2005; 29:1086-94.
- Delia D, Aiello A, Soligo D, Fontanella E, Melani C, Pezzella F, et al. bcl-2 proto-oncogene expression in normal and neoplastic human myeloid cells. Blood 1992; 79:1291-8.
Check Your Price & Order Online
| Product | Size | | IR614 RTU, FLEX
| 60 tests, 12 mL Product No. IR61461-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Tonsil | 120 tests, 24 mL Product No. IR75066-2 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021-2 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human BCL2 Oncoprotein, Clone 124, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels cells expressing BCL2 oncoprotein. Positive results aid in the classification of follicular lymphomas and various diffuse lymphoproliferative diseases (1). The antibody is a useful tool in the differential diagnosis of Burkitt lymphoma/leukaemia versus diffuse large B-cell lymphoma (2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human BCL2 Oncoprotein, Clone 124, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels cells expressing BCL2 oncoprotein. Positive results aid in the classification of follicular lymphomas and various diffuse lymphoproliferative diseases (1). The antibody is a useful tool in the differential diagnosis of Burkitt lymphoma/leukaemia versus diffuse large B-cell lymphoma (2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Synthetic peptide comprising amino acids 41-54 of human BCL2 oncoprotein (1).
Specificity
In Western blotting of extracts of normal human spleen (1, 6), t(14;18)-positive follicular lymphoma (1), and myloid leukemic cell lines (6) the antibody labels solely a band of 26 kDa, corresponding to BCL2 oncoprotein under both nonreducing (1) and reducing conditions (1, 6). The antibody labels the myeloid leukemic cell lines, HL-60 (promyelocytic), KG1 (myeloblastic), GM-1 (monoblastic) and K562 (erythromyeloid) (6).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: 124 (1). Isotype: IgG1, kappa.
Clone: 124 (1). Isotype: IgG1, kappa.
References
- Pezzella F, Tse AGD, Cordell JL, Pulford KAF, Gatter KC, Mason DY. Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation. Am J Pathol 1990; 137:225-32.
- Haralambieva E, Boerma E-J, van Imhoff GW, Rosati S, Schuuring E, Müller-Hermelink HK, et al. Clinical, immunophenotypic, and genetic analysis of adult lymphomas with morphologic features of Burkitt lymphoma. Am J Surg Pathol 2005; 29:1086-94.
- Delia D, Aiello A, Soligo D, Fontanella E, Melani C, Pezzella F, et al. bcl-2 proto-oncogene expression in normal and neoplastic human myeloid cells. Blood 1992; 79:1291-8.
Check Your Price & Order Online
| Product | Size | | IS614 RTU, FLEX
| 30 tests, 6 mL Product No. IS61430-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Tonsil | 60 tests, 12 mL Product No. IS75061-2 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021-2 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |
Synonym(s) for Antigen
oncoprotein, BCL2