United States of America
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Monoclonal Mouse Anti-
Bromodeoxyuridine
Clone Bu20a
Binds to cells which have incorporated bromodeoxyuridine into their DNA during the S-phase of the cell cycle.
Isotype IgG1, kappa
- Frozen
- Formalin
- HIER
- Excellent specificity
- High lot to lot consistency
- For immunohistochemistry (IHC)
- Certified manufacturing facilities guarantee full quality control
Intended Use
For research use only. Not for use in diagnostic procedures.
Monoclonal Mouse Anti-Bromodeoxyuridine, Clone Bu20a, is recommended for use in immunocytochemistry and flow cytometry for identification of cells that have bromodeoxyuridine incorporated in their DNA.
Monoclonal Mouse Anti-Bromodeoxyuridine, Clone Bu20a, is recommended for use in immunocytochemistry and flow cytometry for identification of cells that have bromodeoxyuridine incorporated in their DNA.
Immunogen
Bromodeoxyuridine conjugated to bovine serum albumin.
Specificity
Anti-BrdU, Clone Bu20a, shows, in common with other monoclonal antibodies of this specificity, weak cross-reactivity with other nucleosides, e.g. thymidine (3, 4). However, the antibody has been specifically selected with regard to minimal cross-reactivity.
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: Bu20a (2). Isotype: IgG1, kappa.
Mouse IgG concentration mg/L: See label on vial.
Clone: Bu20a (2). Isotype: IgG1, kappa.
Mouse IgG concentration mg/L: See label on vial.
References
- Magaud JP, Sargent I, Clarke PJ, Ffrench M, Rimokh R, Mason DY. Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem 1989;37:1517-27.
- Vanderlaan M, Thomas CB. Characterization of monoclonal antibodies to bromodeoxyuridine. Cytometry 1985;6:501-5.
- Niedobitek G, Finn T, Herbst H, Bornhöft G, Gerdes J, Stein H. Detection of viral DNA by in situ hybridization using bromodeoxyuridine-labeled DNA probes. Am J Pathol 1988;131:1-4.
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| Product | Size |  | M0744 Culture supernatant
| 1 mL Product No. M074401-8 |
|---|
Synonym(s) for Antigen
BrdU