Monoclonal Mouse Anti-Human

CD99, MIC2 Gene Products, Ewing's Sarcoma Marker

Clone 12E7

The MIC2 gene products are also called p30/32mic2.

They are expressed on the cell membrane of some lymphocytes (bone marrow, lymph nodes and spleen), cortical thymocytes, granulosa cells of the ovary, most Langerhans' islet cells, CNS ependymal cells, Sertoli's cells of the testis and, in a few cases, endothelial cells of single blood vessels. Among neoplastic tissues, the MIC2 gene products are most strongly expressed on the cell membrane of Ewing's sarcoma and primitive peripheral neuroectoderm tumors.

Isotype IgG1, kappa

  • Frozen
  • Formalin

Ewing's sarcoma (FFPE) stained with FLEX Anti-CD99, Code IR057/IS057.

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Ewing's sarcoma. Most tumor cells show a strong membranous and cytoplasmic staining reaction.

Clinical application for IR057/IS057. For identification of glioblastomas and ependymomas of the CNS, certain islet cell tumors of the pancreas, Ewing's sarcoma, granulosa cell tumor and primitive peripheral neuroectodermal tumors.

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Granulosa cell tumor. The neoplastic cells show a weak to moderate membranous and cytoplasmic staining reaction.

Clinical application for IR057/IS057. For identification of glioblastomas and ependymomas of the CNS, certain islet cell tumors of the pancreas, Ewing's sarcoma, granulosa cell tumor and primitive peripheral neuroectodermal tumors.

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Pancreas. The pancreatic islet cells show a moderate to strong cytoplasmic staining reaction.

Recommended control for IR057/IS057. Pancreas/Tonsil/Thymus.

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Tonsil. The squamous epithelial cells show a weak to moderate membranous staining reaction. The lymphocytes should a show a weak to moderate and predominantly membranous staining reaction.

Recommended control for IR057/IS057. Pancreas/Tonsil/Thymus.

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Ewing's sarcoma stained with Anti-CD99.

Clinical application for IR057/IS057. For identification of glioblastomas and ependymomas of the CNS, certain islet cell tumors of the pancreas, Ewing's sarcoma, granulosa cell tumor and primitive peripheral neuroectodermal tumors.

Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.

  • Excellent specificity
  • High lot to lot consistency
  • Optimized for immunohistochemistry (IHC) with validated protocols
  • Certified manufacturing facilities guarantee full quality control
Dako's products are the result of 40 years experience ensuring quality and innovation in antibodies and reagents: the genesis of the pathology lab of tomorrow.

Intended Use
For in vitro diagnostic use.

Monoclonal Mouse Anti-Human CD99, MIC2 Gene Product, Ewing's Sarcoma Marker, Clone 12E7 is intended for laboratory use to identify qualitatively by light microscopy CD99, MIC2 gene product in normal and malignant cells using immunohistochemical (IHC) test methods. Positive results aid in the classification of tumors of the lung and thyroid. The clinical interpretation of any positive staining or its absence should be complemented by morphological and histological studies with proper controls. Evaluations should be made within the context of the patient's clinical history and other diagnostic tests by a qualified individual.
Immunogen
Acute lymphocytic leukemia T-cells (1).
Specificity
MIC2, 12E7 recognizes the products of the MIC2 gene and has been shown to have similar or identical reactivity to monoclonal antibodies HBA-71 and RFB-1 (3, 6).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as tissue culture supernatant in 0.05 mol/L Tris-HCl, pH 7.2 and 0.015 mol/L sodium azide. This product contains stabilizing protein.

Clone: 12E7 (1). Isotype: IgG1, kappa.

Mouse lgG concentration mg/L: See label on vial.

The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
References
  1. Levy R, Dilley J, Fox RI, Warnke R. A human thymus-leukemia antigen defined by hybridoma monoclonal antibodies. PNAS USA 1979;76(12):6552-6
  1. Latron F, Blanchard D, Cartron JP. Immunochemical characterization of the human blood cell membrane glycoprotein recognized by the monoclonal antibodies 12E7. Biochem J 1987;247(3):757-64
  1. Ambros IM, Ambros PF, Strehl S, Kovar H, Gadner H, Salzer-Kuntschik M. MIC2 is a specific marker for Ewing's sarcoma and peripheral primitive neuroectodermal tumors. Cancer 1991;67(1):1886-93
ProductSize
For In Vitro Diagnostic UseM3601 Culture supernatant
Specification Sheet
Safety Data Sheet:
Non-hazard classification statement
1 mL
Product No. M360101-2

  • Optimized staining performance of both high- and low-expression structures
  • Crisp and clear staining with no background
  • Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy

Intended Use
For in vitro diagnostic use.

FLEX Monoclonal Mouse Anti-Human CD99, MIC2 Gene Product, Ewing's Sarcoma Marker, Clone 12E7, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of glioblastomas and ependymomas of the CNS, certain islet cell tumors of the pancreas, particularly Ewing's Sarcoma (ES), and primitive peripheral neuroectodermal tumors (pPNET). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Acute lymphocytic leukemia T-cells (1)
Specificity
The antibody recognizes the products of the MIC2 gene and has been shown to have similar or identical reactivity to monoclonal antibodies HBA-71 and RFB-1 (2,5).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.

Clone: 12E7. Isotype: IgG1, kappa.
References
  1. Levy R, et al. A human thymus-leukemia antigen defined by hybridoma monoclonal antibodies. PNAS USA 1979; 76:6552
  2. Latron F, et al. Immunochemical characterization of the human blood cell membrane glycoprotein recognized by the monoclonal antibodies 12E7. Biochem J 1987; 247:757
  1. Ambros IM, et al. MIC2 is a specific marker for Ewing's sarcoma and peripheral primitive neuroectodermal tumors. Cancer 1991; 67:1886
ProductSize
For In Vitro Diagnostic UseIR057 RTU, FLEX
Specification Sheet
Safety Data Sheet:
Non-hazard classification statement
60 tests, 12 mL
Product No. IR05761-2

RECOMMENDED COMPANION PRODUCTS

StepProductSize
Pre-treatmentEnVision™ FLEX Target Retrieval Solution, High pH (Code K8004)
3-in-1 procedure using PT Link
3 x 30 mL
Product No. K800421-2
Antibody Dilution
Ready-to-use / Prediluted
 
Negative ControlFLEX Negative Control, Mouse (Code IR750)
Positive tissue: Thymus (cortical thymocytes)
120 tests, 24 mL
Product No. IR75066-2
Visualization SystemEnVision™ FLEX (Code K8000)
Use High pH Target Retrieval Solution
400-600 tests
Product No. K800021-2
CounterstainHematoxylin (Link) (Code K8008)
Non-aqueous, permanent mounting medium is required
3 x 45 mL, 400-600 tests
Product No. K800821-2
Glass SlidesSilanized Slides (Code S3003)
Recommended for greater adherence
Alternative is FLEX IHC Microscope Slides (Code K8020)
100 slides
Product No. S300330-2

  • Optimized staining performance of both high- and low-expression structures
  • Crisp and clear staining with no background
  • Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy

Intended Use
For in vitro diagnostic use.

FLEX Monoclonal Mouse Anti-Human CD99, MIC2 Gene Product, Ewing's Sarcoma Marker, Clone 12E7, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of glioblastomas and ependymomas of the CNS, certain islet cell tumors of the pancreas, particularly Ewing's Sarcoma (ES), and primitive peripheral neuroectodermal tumors (pPNET). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Acute lymphocytic leukemia T-cells (1)
Specificity
The antibody recognizes the products of the MIC2 gene and has been shown to have similar or identical reactivity as monoclonal antibodies HBA-71 and RFB-1 (2,5).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.

Clone: 12E7. Isotype: IgG1, kappa.
References
  1. Levy R, et al. A human thymus-leukemia antigen defined by hybridoma monoclonal antibodies. PNAS USA 1979; 76:6552
  2. Latron F, et al. Immunochemical characterization of the human blood cell membrane glycoprotein recognized by the monoclonal antibodies 12E7. Biochem J 1987; 247:757
  1. Ambros IM, et al. MIC2 is a specific marker for Ewing's sarcoma and peripheral primitive neuroectodermal tumors. Cancer 1991; 67:1886
ProductSize
For In Vitro Diagnostic UseIS057 RTU, FLEX
Specification Sheet
Safety Data Sheet:
Non-hazard classification statement
30 tests, 6 mL
Product No. IS05730-2

RECOMMENDED COMPANION PRODUCTS

StepProductSize
Pre-treatmentEnVision™ FLEX Target Retrieval Solution, High pH (Code K8004)
3-in-1 procedure using PT Link
3 x 30 mL
Product No. K800421-2
Antibody Dilution
Ready-to-use / Prediluted
 
Negative ControlFLEX Negative Control, Mouse (Code IS750)
Positive tissue: Thymus (cortical thymocytes)
60 tests, 12 mL
Product No. IS75061-2
Visualization SystemEnVision™ FLEX (Code K8010)
Use High pH Target Retrieval Solution
400-600 tests
Product No. K801021-2
CounterstainHematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018)
Non-aqueous, permanent mounting medium is required
10 x 13 mL, 400-600 tests
Product No. K801821-2
Glass SlidesSilanized Slides (Code S3003)
Recommended for greater adherence
Alternative is FLEX IHC Microscope Slides (Code K8020)
100 slides
Product No. S300330-2

Synonym(s) for Antigen

Ewing's sarcoma marker, MIC2 gene product, p30/32mic2

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