LAST PRODUCTS VIEWED
Monoclonal Mouse Anti-Human
CD105, Endoglin
Clone SN6h
Endoglin is a type I transmembrane protein which is highly expressed on human vascular endothelial cells. A large variety of tissues express endoglin. It is recommended to use a highly sensitive visualization system when the antibody is used on formalin-fixed tissue.
Isotype IgG1, kappa
- Frozen
- Formalin
- Enzyme
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Endoglin is a Type I transmembrane protein which is highly expressed on human vascular endothelial cells. Up-regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation-associated endothelial cell marker (2,3). Endoglin exists as an O- and N-glycosylated homodimer of 95 kD subunits and was designated CD105 at the Fifth International Workshop on Leucocyte Differentiation Antigens (Boston) (4). The clinical interpretation of any positive staining or its absence should be complemented by morphological and histological studies with proper controls. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Endoglin is a Type I transmembrane protein which is highly expressed on human vascular endothelial cells. Up-regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation-associated endothelial cell marker (2,3). Endoglin exists as an O- and N-glycosylated homodimer of 95 kD subunits and was designated CD105 at the Fifth International Workshop on Leucocyte Differentiation Antigens (Boston) (4). The clinical interpretation of any positive staining or its absence should be complemented by morphological and histological studies with proper controls. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Purified CP160 from cell membrane glycoproteins of fresh non-T/non-B acute lymphoblastic leukemia cells (1).
Specificity
Endoglin has been demonstrated to be a component of the transforming growth factor (TGF) βreceptor system in human umbilical vein endothelial cells and binds TGFβ1 and β3 with high affinity, but not TGFβ2 (4,5). Monoclonal mouse anti-endoglin, SN6h, was originally described as reacting with GP160, a human leukemia-associated cell surface glycoprotein (1,6), which was later identified as endoglin (7).
Reagent Provided
Anti-endoglin, SN6h, is a mouse monoclonal antibody supplied in liquid form as tissue culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris-HCl, pH 7.2 and 0.015 mol/L sodium azide. Contains stabilizing protein.
Clone: SN6h (1). Isotype: IgG1, kappa
Mouse lgG concentration mg/L: See label on vial.
Clone: SN6h (1). Isotype: IgG1, kappa
Mouse lgG concentration mg/L: See label on vial.
References
- Haruta Y, et al. Multiple epitopes of GP160, a novel human leukemia-associated cell surface glycoprotein. Fed Proc 1987; 46(3):1056
- Burrows FJ, et al. Up-regulation of endoglin on vascular endothelial cells in human solid tumors: Implications for diagnosis and therapy. Clin Canc Res 1995; 1:1623
- Matsuzaki H, et al. Effect of induced transformation of human leukemia cells on the expression of GP160, a novel human leukemia-associated cell surface glycoprotein. Fed Proc 1987; 46(3):1056
- Letarte M, et al. CD105 (endoglin) cluster report. In Schlossman SF, et al (eds). Leucocyte Typing V White Cell Differentiation Antigens. Oxford University Press (Oxford) 1995; 1756
- Cheifetz S, et al. Endoglin is a component of the transforming growth factor-beta receptor system in human endothelial cells. J Biol Chem 1992; 267(27):19027
- Haruta Y and Seon BK. Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6. Proc Natl Acad Sci USA 1986; 83:7898
- Seon BK, et al. Multiple epitopes of human endoglin, transforming growth factor-β receptor III, detected by SN6 series monoclonal antibodies. Proc Amer Assoc Canc Res 1995; 36:23
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| Product | Size |  | M3527 Culture supernatant
| 1 mL Product No. M352701-2 |
|---|
Synonym(s) for Antigen
endoglin, GP160