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Monoclonal Mouse Anti-Human
CD138
Clone MI15
CD138, syndecan-1, is a transmembrane proteoglycan with a main cellular expression in stratified and simple epithelia.
Within the hemopoietic system, CD138 is mainly confined to late stages of B-cell differentiation. CD138 expression is reduced during malignant transformation of various epithelia, and CD138 is rapidly shed by myeloma cells entering into apoptosis, making CD138 a marker of viable myeloma cells. This antibody is useful for the identification of malignancies involving terminally differentiated plasma cells, and CD138 is associated with multiple myeloma. Anti-CD138 may also be useful for the subclassification of diffuse large B-cell lymphomas.
Isotype IgG1, kappa
- Frozen
- Formalin
- HIER
Diffuse large B-cell lymphoma (FFPE) stained with FLEX Anti-CD138, Code IR642/IS642.
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Diffuse large B-cell lymphoma. The neoplastic cells show a distinct membranous staining reaction.
Clinical application for IR642/IS642. For identification of malignancies involving terminally differentiated plasma cells and multiple myeloma, and for subclassification of diffuse large B-cell lymphoma.
Myeloma. The majority of the neoplastic cells show a strong and distinct membranous staining reaction.
Clinical application for IR642/IS642. For identification of malignancies involving terminally differentiated plasma cells and multiple myeloma, and for subclassification of diffuse large B-cell lymphoma.
Tonsil. The majority of the plasma cells show a moderate to strong predominantly membranous staining reaction. Scattered stimulated immunoblasts show a moderate to strong staining reaction, while the majority of the germinal center cells and peripheral lymphocytes should be negative.
Recommended control for IR642/IS642. Appendix/Colon/Tonsil.
Appendix. The plasma cells in lamina propria and the epithelial cells show a moderate to strong predominantly membranous staining reaction. The luminal and basal epithelial cells should be demonstrated.
Recommended control for IR642/IS642. Appendix/Colon/Tonsil.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human CD138, Clone MI15, is intended for use in immunohistochemistry together with a panel of other antibodies. CD138 is present in malignancies involving terminally differentiated plasma cells, and it is associated with multiple myeloma (1, 2, 3, 4). Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Monoclonal Mouse Anti-Human CD138, Clone MI15, is intended for use in immunohistochemistry together with a panel of other antibodies. CD138 is present in malignancies involving terminally differentiated plasma cells, and it is associated with multiple myeloma (1, 2, 3, 4). Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Immunogen
A mixture of U266 and XG-1 human myeloma cell lines (3, 9).
Specificity
Anti-CD138, MI15, was included in the Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens (Kobe 1996), and studies by a number of different laboratories confirmed its reactivity with CD138 (1).
The epitope recognized by anti-CD138, MI15, is found within the ectodomain (extracellular part) of the CD138 core protein. Blocking experiments have revealed that the epitope recognized by MI15 seems to be partly overlapping the epitope recognized by another antibody to CD138, clone B-B4 (1, 5). Anti-CD138, MI15, reacts strongly with CD138 expressed by multiple myeloma-derived cell lines, such as U266 and XG-1 (9).
The epitope recognized by anti-CD138, MI15, is found within the ectodomain (extracellular part) of the CD138 core protein. Blocking experiments have revealed that the epitope recognized by MI15 seems to be partly overlapping the epitope recognized by another antibody to CD138, clone B-B4 (1, 5). Anti-CD138, MI15, reacts strongly with CD138 expressed by multiple myeloma-derived cell lines, such as U266 and XG-1 (9).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: MI15. Isotype: IgG1, kappa.
Mouse IgG concentration: See label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
Clone: MI15. Isotype: IgG1, kappa.
Mouse IgG concentration: See label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
References
- Wijdenes J, Clément C, Klein B, Dore J-M. New B-cell CD antigens. BC29: CD138 (syndecan-1) workshop panel report. In: Kishimoto T, Kikutani H, von dem Borne AEG, Goyert SM, Mason DY, Miyasaka M, et al., editors. Leucocyte typing VI. White cell differentiation antigens. Proceedings of the 6th International Workshop and Conference; 1996 Nov 10-14; Kobe, Japan. New York, London: Garland Publishing Inc.; 1997. p. 249-52.
- Jourdan M, Ferlin M, Legouffe E, Horvathova M, Liautard J, Rossi JF, et al. The myeloma cell antigen syndecan-1 is lost by apoptotic myeloma cells. Br J Haematol 1998;100:637-46.
- Costes V, Magen V, Legouffe E, Durand L, Baldet P, Rossi J-F, et al. The MI15 monoclonal antibody (anti-syndecan-1) is a reliable marker for quantifying plasma cells in paraffin-embedded bone marrow biopsy specimens. Hum Pathol 1999;30:1405-11.
- van Zaanen HCT, Vet RJWM, de Jong CM, von dem Borne AEGKr, van Oers MHJ. A simple and sensitive method for determining plasma cell isotype and monoclonality in bone marrow using flow cytometry. Br J Haematol 1995;91:55-9.
- Gattei V, Godeas C, Degan M, Rossi FM, Aldinucci D, Pinto A. Characterization of anti-CD138 monoclonal antibodies as tools for investigating the molecular polymorphism of syndecan-1 in human lymphoma cells. Br J Haematol 1999;104:152-62.
- Horvathova M, Gaillard JP, Liautard J, Duperray C, Lavabre-Bertrand T, Bourquard P, et al. B30.6. Identification of novel and specific antigens of human plasma cells by mAb. In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, et al., editors. Leucocyte typing V. White cell differentiation antigens. Proceedings of the 5th International Workshop and Conference; 1993 Nov 3-7; Boston, USA. Oxford, New York, Tokyo: Oxford University Press; 1995. p. 713-4.
Check Your Price & Order Online
| Product | Size |  | M7228 Culture supernatant
| 1 mL Product No. M722801-2 |
|---|
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human CD138, Clone MI15, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of malignancies involving terminally differentiated plasma cells, and it is associated with multiple myeloma (1, 2). Antibodies to CD138 may also be useful for the subclassification of diffuse large B-cell lymphoma (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human CD138, Clone MI15, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of malignancies involving terminally differentiated plasma cells, and it is associated with multiple myeloma (1, 2). Antibodies to CD138 may also be useful for the subclassification of diffuse large B-cell lymphoma (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
A mixture of U266 and XG-1 human myeloma cell lines (2, 9).
Specificity
Anti-Human CD138, clone MI15, was clustered as anti-CD138 at the 6th International Workshop and Conference on Human Leucocyte Differentiation Antigens (1).
The epitope recognized by anti-CD138, MI15, is found within the ectodomain (extracellular part) of the CD138 core protein. Blocking experiments have revealed that the epitope recognized by MI15 seems to be partly overlapping the epitope recognized by another antibody to CD138, clone B-B4 (1, 5). Anti-CD138, MI15, reacts strongly with CD138 expressed by multiple myeloma-derived cell lines, such as U266 and XG-1 (9).
The epitope recognized by anti-CD138, MI15, is found within the ectodomain (extracellular part) of the CD138 core protein. Blocking experiments have revealed that the epitope recognized by MI15 seems to be partly overlapping the epitope recognized by another antibody to CD138, clone B-B4 (1, 5). Anti-CD138, MI15, reacts strongly with CD138 expressed by multiple myeloma-derived cell lines, such as U266 and XG-1 (9).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: MI15. Isotype: IgG1, kappa.
Clone: MI15. Isotype: IgG1, kappa.
References
- Wijdenes J, Clément C, Klein B, Dore J-M. New B-cell CD antigens. BC29: CD138 (syndecan-1) workshop panel report. In: Kishimoto T, Kikutani H, von dem Borne AEG, Goyert SM, Mason DY, Miyasaka M, et al., editors. Leucocyte typing VI. White cell differentiation antigens. Proceedings of the 6th International Workshop and Conference; 1996 Nov 10-14; Kobe, Japan. New York, London: Garland Publishing Inc.; 1997. p. 249-52.
- Costes V, Magen V, Legouffe E, Durand L, Baldet P, Rossi J-F, et al. The MI15 monoclonal antibody (anti-syndecan-1) is a reliable marker for quantifying plasma cells in paraffin-embedded bone marrow biopsy specimens. Hum Pathol 1999;30:1405-11.
- Colomo L, López-Guillermo A, Perales M, Rives S, Martínez A, Bosch F, et al. Clinical impact of the differentiation profile assessed by immunophenotyping in patients with diffuse large B-cell lymphoma. Blood 2003;101:78-84.
- Horvathova M, Gaillard JP, Liautard J, Duperray C, Lavabre-Bertrand T, Bourquard P, et al. B30.6. Identification of novel and specific antigens of human plasma cells by mAb. In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, et al., editors. Leucocyte typing V. White cell differentiation antigens. Proceedings of the 5th International Workshop and Conference; 1993 Nov 3-7; Boston, USA. Oxford, New York, Tokyo: Oxford University Press; 1995. p. 713-4.
Check Your Price & Order Online
| Product | Size | | IR642 RTU, FLEX
| 60 tests, 12 mL Product No. IR64261-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Tonsil | 120 tests, 24 mL Product No. IR75066-2 |
| Visualization System | EnVision™ FLEX+ (Code K8002) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800221-2 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821-2 |
| Glass Slides | Silanized Slides (Code S3003) Recommended for greater adherence Alternative is FLEX IHC Microscope Slides (Code K8020) | 100 slides Product No. S300330-2 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human CD138, Clone MI15, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of malignancies involving terminally differentiated plasma cells, and it is associated with multiple myeloma (1, 2). Antibodies to CD138 may also be useful for the subclassification of diffuse large B-cell lymphoma (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human CD138, Clone MI15, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of malignancies involving terminally differentiated plasma cells, and it is associated with multiple myeloma (1, 2). Antibodies to CD138 may also be useful for the subclassification of diffuse large B-cell lymphoma (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
A mixture of U266 and XG-1 human myeloma cell lines (2, 9).
Specificity
Anti-Human CD138, clone MI15, was clustered as anti-CD138 at the 6th International Workshop and Conference on Human Leucocyte Differentiation Antigens (1).
The epitope recognized by anti-CD138, MI15, is found within the ectodomain (extracellular part) of the CD138 core protein. Blocking experiments have revealed that the epitope recognized by MI15 seems to be partly overlapping the epitope recognized by another antibody to CD138, clone B-B4 (1, 5). Anti-CD138, MI15, reacts strongly with CD138 expressed by multiple myeloma-derived cell lines, such as U266 and XG-1 (9).
The epitope recognized by anti-CD138, MI15, is found within the ectodomain (extracellular part) of the CD138 core protein. Blocking experiments have revealed that the epitope recognized by MI15 seems to be partly overlapping the epitope recognized by another antibody to CD138, clone B-B4 (1, 5). Anti-CD138, MI15, reacts strongly with CD138 expressed by multiple myeloma-derived cell lines, such as U266 and XG-1 (9).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: MI15. Isotype: IgG1, kappa.
Clone: MI15. Isotype: IgG1, kappa.
References
- Wijdenes J, Clément C, Klein B, Dore J-M. New B-cell CD antigens. BC29: CD138 (syndecan-1) workshop panel report. In: Kishimoto T, Kikutani H, von dem Borne AEG, Goyert SM, Mason DY, Miyasaka M, et al., editors. Leucocyte typing VI. White cell differentiation antigens. Proceedings of the 6th International Workshop and Conference; 1996 Nov 10-14; Kobe, Japan. New York, London: Garland Publishing Inc.; 1997. p. 249-52.
- Costes V, Magen V, Legouffe E, Durand L, Baldet P, Rossi J-F, et al. The MI15 monoclonal antibody (anti-syndecan-1) is a reliable marker for quantifying plasma cells in paraffin-embedded bone marrow biopsy specimens. Hum Pathol 1999;30:1405-11.
- Colomo L, López-Guillermo A, Perales M, Rives S, Martínez A, Bosch F, et al. Clinical impact of the differentiation profile assessed by immunophenotyping in patients with diffuse large B-cell lymphoma. Blood 2003;101:78-84.
- Horvathova M, Gaillard JP, Liautard J, Duperray C, Lavabre-Bertrand T, Bourquard P, et al. B30.6. Identification of novel and specific antigens of human plasma cells by mAb. In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, et al., editors. Leucocyte typing V. White cell differentiation antigens. Proceedings of the 5th International Workshop and Conference; 1993 Nov 3-7; Boston, USA. Oxford, New York, Tokyo: Oxford University Press; 1995. p. 713-4.
Check Your Price & Order Online
| Product | Size | | IS642 RTU, FLEX
| 30 tests, 6 mL Product No. IS64230-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Tonsil | 60 tests, 12 mL Product No. IS75061-2 |
| Visualization System | EnVision™ FLEX+ (Code K8012) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801221-2 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821-2 |
| Glass Slides | Silanized Slides (Code S3003) Recommended for greater adherence Alternative is FLEX IHC Microscope Slides (Code K8020) | 100 slides Product No. S300330-2 |
Synonym(s) for Antigen
syndecan-1