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Polyclonal Rabbit Anti-Human
Chromogranin A
The antigen used for immunization is the C-terminal half (Mr 23000) of chromogranin A isolated from urine of patients with carcinoid tumors.
A reference to the use of anti-chromogranin in the study of tumors of neuroendocrine origin is (1).
- Formalin
- HIER
References
- Kimura N, Sasano N, Yamada R, Satoh J. Immunohistochemical study of chromogranin in 100 cases of pheochromocytoma, carotid body tumour, medullary thyroid carcinoma, and carcinoid tumour. Virch Arch [A] 1988;413:33-8.
Medullary thyroid carcinoma (FFPE) stained with FLEX Anti-Chromogranin A, Code IR502/IS502.
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Medullary thyroid carcinoma. The majority of the neoplastic cells show a distinct cytoplasmic staining reaction.
Clinical application for IR502/IS502. For identification of neuroendocrine cells and tumors with neuroendocrine differentiation.
Small cell lung carcinoma. The majority of the neoplastic cells show a finely granular, distinct cytoplasmic and focally dot-like staining reaction.
Clinical application for IR502/IS502. For identification of neuroendocrine cells and tumors with neuroendocrine differentiation.
Colon. The neuroendocrine cells in the epithelial surface show a moderate to strong coarse cytoplasmic staining reaction, whereas the epithelial cells are negative.
Recommended control for IR502/IS502. Appendix/Colon/Brain.
Colon. The axons and perikarya of the neurons and ganglion cells in the submucosa and in the tunica muscularis show a weak to moderate granular cytoplasmic staining reaction. No staining of the surrounding muscle cells
Recommended control for IR502/IS502. Appendix/Colon/Brain.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Polyclonal Rabbit Anti-Human Chromogranin A, Code A0430, is intended for the determination of chromogranin A in immunohistochemistry (1). The antibody labels chromogranin A present in secretory granules of endocrine cells. Chromogranin A is a marker for neuroendocrine differentiation and antibodies to chromogranin A are useful for identification of neuroendocrinederived tumors. Differential identification is aided by the results from a panel of antibodies. Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Polyclonal Rabbit Anti-Human Chromogranin A, Code A0430, is intended for the determination of chromogranin A in immunohistochemistry (1). The antibody labels chromogranin A present in secretory granules of endocrine cells. Chromogranin A is a marker for neuroendocrine differentiation and antibodies to chromogranin A are useful for identification of neuroendocrinederived tumors. Differential identification is aided by the results from a panel of antibodies. Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Immunogen
Chromogranin A isolated from urine of patients with carcinoid syndrome.
Specificity
The antibody reacts with human chromogranin A, but not with epitopes located on the N-terminal half of the chromogranin A molecule. The antibody labels the neuroendocrine cells and neural tissue in colon, pancreas and peripheral nerve tissue. No non-specific staining has been observed. Traces of contaminating antibodies have been removed by solid-phase absorption with human urine proteins.
Crossed immunoelectrophoresis: When using 12.5 µL Dako A0430 per square cm gel area against 2 µL human plasma or 2 µL concentrated urine from patients with tubular proteinuria no precipitate is seen.
As demonstrated by immunocytochemistry, the antibody cross-reacts with the chromogranin A-equivalent protein in cow, horse and swine.
Crossed immunoelectrophoresis: When using 12.5 µL Dako A0430 per square cm gel area against 2 µL human plasma or 2 µL concentrated urine from patients with tubular proteinuria no precipitate is seen.
As demonstrated by immunocytochemistry, the antibody cross-reacts with the chromogranin A-equivalent protein in cow, horse and swine.
Reagent Provided
Purified immunoglobulin fraction of rabbit antiserum provided in liquid form. In 0.1 mol/L NaCl, 15 mmol/L NaN3.
Protein concentration g/L: See label on vial.
Protein concentration g/L: See label on vial.
References
- Moorghen M, Carpenter F. Peanut lectin: a histochemical marker for phaeo¬chromocytomas. Virchows Arch A Pathol Anal Histopathol 1991;419:203-7.
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| Product | Size |  | A0430 Ig fraction
| 0.2 mL Product No. A043029-2 |
|---|
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Human Chromogranin A, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels chromogranin A present in secretory granules of endocrine cells (1). Chromogranin A is a marker for neuroendocrine differentiation and antibodies to chromogranin A are useful for identification of neuroendocrine-derived tumors. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Polyclonal Rabbit Anti-Human Chromogranin A, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels chromogranin A present in secretory granules of endocrine cells (1). Chromogranin A is a marker for neuroendocrine differentiation and antibodies to chromogranin A are useful for identification of neuroendocrine-derived tumors. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Chromogranin A isolated from urine of patients with carcinoid syndrome.
Specificity
The antibody reacts with human chromogranin A, but not with epitopes located on the N-terminal half of thechromogranin A molecule. The antibody labels the neuroendocrine cells and neural tissue in colon, pancreas and peripheral nerve tissue. No non-specific staining has been observed. Traces of contaminating antibodies have been removed by solid-phase absorption with human urine proteins.
As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections, the antibody cross-reacts with the chromogranin A-equivalent protein in cow, horse and swine.
As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections, the antibody cross-reacts with the chromogranin A-equivalent protein in cow, horse and swine.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
References
- Moorghen M, Carpenter F. Peanut lectin: a histochemical marker for phaeochromocytomas. Virchows Arch A Pathol Anal Histopathol 1991; 419:203-7.
Check Your Price & Order Online
| Product | Size | | IR502 RTU, FLEX
| 60 tests, 12 mL Product No. IR50261-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IR600) Positive tissue: Colon | 120 tests, 24 mL Product No. IR60066-2 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021-2 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Human Chromogranin A, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels chromogranin A present in secretory granules of endocrine cells (1). Chromogranin A is a marker for neuroendocrine differentiation and antibodies to chromogranin A are useful for identification of neuroendocrine-derived tumors. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Polyclonal Rabbit Anti-Human Chromogranin A, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels chromogranin A present in secretory granules of endocrine cells (1). Chromogranin A is a marker for neuroendocrine differentiation and antibodies to chromogranin A are useful for identification of neuroendocrine-derived tumors. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Chromogranin A isolated from urine of patients with carcinoid syndrome.
Specificity
The antibody reacts with human chromogranin A, but not with epitopes located on the N-terminal half of the chromogranin A molecule. The antibody labels the neuroendocrine cells and neural tissue in colon, pancreas and peripheral nerve tissue. No non-specific staining has been observed. Traces of contaminating antibodies have been removed by solid-phase absorption with human urine proteins.
As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections, the antibody cross-reacts with the chromogranin A-equivalent protein in cow, horse and swine.
As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections, the antibody cross-reacts with the chromogranin A-equivalent protein in cow, horse and swine.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
References
- Moorghen M, Carpenter F. Peanut lectin: a histochemical marker for phaeochromocytomas. Virchows Arch A Pathol Anal Histopathol 1991; 419:203-7.
Check Your Price & Order Online
| Product | Size | | IS502 RTU, FLEX
| 30 tests, 6 mL Product No. IS50230-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IS600) Positive tissue: Colon | 60 tests, 12 mL Product No. IS60061-2 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021-2 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |