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Polyclonal Rabbit Anti-Human
Kappa Light Chains
These reagents have been produced in a manner that ensures a particularly wide specificity for kappa-chains.
The specificity is directed against surface as well as hidden determinants and has been ascertained by gel precipitation techniques and immunohistochemistry. This antibody is useful for the identification of plasma cells and related lymphoid cells containing kappa light chains, and it is a useful tool for the classification of patients with monoclonal gammopathies and amyloidosis. Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of B cells.
- Frozen
- Formalin
- Enzyme/HIER
B-cell lymphoma (FFPE) stained with FLEX Anti-Kappa Light Chains, Code IR506/IS506.
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B-cell lymphoma. Most neoplastic cells show a distinct membranous and cytoplasmic staining reaction.
Clinical application for IR506/IS506 For identification of plasma cells and lymphoid cells containing kappa light chains, and for classification of kappa light chain restriction for monoclonal gammopathies and amyloidosis.
Plasmacytoma. The tumor cells show a strong cytoplasmic staining reaction.
Clinical application for IR506/IS506 For identification of plasma cells and lymphoid cells containing kappa light chains, and for classification of kappa light chain restriction for monoclonal gammopathies and amyloidosis.
Tonsil. B cells in the mantle zone show a weak to moderate membranous staining reaction, while the B lymphocytes in the germinal center are weakly demonstrated. Plasma cells and immunoblasts in the germinal center show a moderate to strong cytoplasmic staining.
Recommended control for IR506/IS506. Tonsil.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
F0198 is intended for demonstration of kappa light chains in tissues, and may also be used for other immunofluorescencetechniques (1-3). Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
F0198 is intended for demonstration of kappa light chains in tissues, and may also be used for other immunofluorescencetechniques (1-3). Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Immunogen
Polyclonal immunoglobulin light chains of kappa type isolated from a pool of human sera.
Specificity
F0198 labels free kappa chains as well as kappa chains in intact immunoglobulin molecules. Traces of contaminating antibodies have been removed by solid-phase absorption.
In crossed immunoelectrophoresis, only the kappa precipitation arch appears when using 37.5 µL antibody per cm2 gelarea against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
In crossed immunoelectrophoresis, only the kappa precipitation arch appears when using 37.5 µL antibody per cm2 gelarea against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
Reagent Provided
The Anti-Human Kappa Light Chains conjugate, F0198, has been produced from a purified immunoglobulin fraction ofrabbit antiserum. F0198 is provided in liquid form in phosphate buffer containing 15 mmol/L NaN3, pH 7.2.
Protein concentration g/L: See label on vial.
Antibody titre: 200 mg/L (mg of antigen to be added to 1 L of antibody to reach equivalence point).
F/P ratio: E495 nm/E278 nm = 0.65 ± 0.05 corresponding to a molar FITC/protein ratio of 2.5.
Protein concentration g/L: See label on vial.
Antibody titre: 200 mg/L (mg of antigen to be added to 1 L of antibody to reach equivalence point).
F/P ratio: E495 nm/E278 nm = 0.65 ± 0.05 corresponding to a molar FITC/protein ratio of 2.5.
References
- Muda AO, Feriozzi S, Rahmi S, Faraggiana T. Spatial arrangement of IgA and C3 as a prognostic indicator of IgAnephopathy. J Pathol 1995;177:201-8.
- Rhodes JM, Jewell DP, Janossy G. Alpha-chain disease diagnosed by rectal biopsy. Brit Med J 1980;5:1043-4.
- Lai K-N, Chan KW, Mac-Moune F, Ho CP, Yan KW, Lam CWK, et al. The immunohistochemical characterizationof the light chains in the mesangial IgA deposits in IgA nephropathy. Am J Clin Pathol 1986;85:548-51.
Check Your Price & Order Online
| Product | Size |  | A0191 Ig fraction
| 2 mL Product No. A019102-2 | | F0198 FITC. Ig fraction
| 2 mL Product No. F019802-2 |
|---|
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Human Kappa Light Chains, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of plasma cells and related lymphoid cells containing kappa light chains, and it is a useful tool for the classification of patients with monoclonal gammopathies (2) and amyloidosis (3, 4). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of B cells (5-7). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Polyclonal Rabbit Anti-Human Kappa Light Chains, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of plasma cells and related lymphoid cells containing kappa light chains, and it is a useful tool for the classification of patients with monoclonal gammopathies (2) and amyloidosis (3, 4). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of B cells (5-7). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Polyclonal light chains of type kappa isolated from pooled human sera.
Specificity
The antibody reacts with free kappa chains as well as kappa chains in intact immuno¬globulin molecules. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
Specificity tests; staining with Coomassie Brilliant Blue:
Double immunodiffusion: No reaction with lambda or gamma chains is seen when using 15 µL of concentrated antibody against 15 µL of lambda IgG.
Crossed immunoelectrophoresis: Only kappa-related precipitates appear when using 12.5 µL concentrated antibody per square cm gel area against 2 µL human plasma or 2 µL of a pool of concentrated urines from patients with tubular proteinuria.
Specificity tests; staining with Coomassie Brilliant Blue:
Double immunodiffusion: No reaction with lambda or gamma chains is seen when using 15 µL of concentrated antibody against 15 µL of lambda IgG.
Crossed immunoelectrophoresis: Only kappa-related precipitates appear when using 12.5 µL concentrated antibody per square cm gel area against 2 µL human plasma or 2 µL of a pool of concentrated urines from patients with tubular proteinuria.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3
References
- Peterson LC, Brown BA, Crosson JT, Mladenovic J. Application of the immunoperoxidase technic to bone marrow trephine biopsies in the classification of patients with monoclonal gammopathies. Am J Clin Pathol 1986;85:688-93.
- Strøm EH, Fogazzi GB, Banfi G, Pozzi C, Mihatsch MJ. Light chain deposition disease of the kidney. Morphological aspects in 24 patients. Virchows Arch 1994;425:271-80.
- Jessup E. Antigen retrieval techniques for the demonstration of immunoglobulin light chains in formalin-fixed, paraffin-wax embedded sections. UK NEQAS immunocytochemistry news. Winter 1994/95; Issue 4:12-6.
- Taylor CR, Burns J. The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody. J Clin Path 1974;27:14-20.
- Harris NL, Poppema S, Data RE. Demonstration of immunoglobulin in malignant lymphomas. Use of an immunoperoxidase technic on frozen sections. Am J Clin Pathol 1982;78:14-21.
- Marshall-Taylor CE, Cartun RW, Mandich D, DiGuiseppe JA. Immunohistochemical detection of immunoglobulin light chain expression in B-cell non-Hodgkin lymphomas using formalin-fixed, paraffin-embedded tissues and a heat-induced epitope retrieval technique. Appl Immunohistochem Mol Morphol 2002;10:258-62.
Check Your Price & Order Online
| Product | Size | | IR506 RTU, FLEX
| 60 tests, 12 mL Product No. IR50661-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IR600) Positive tissue: Tonsil | 120 tests, 24 mL Product No. IR60066-2 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021-2 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Human Kappa Light Chains, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of plasma cells and related lymphoid cells containing kappa light chains, and it is a useful tool for the classification of patients with monoclonal gammopathies (2) and amyloidosis (3, 4). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of B cells (5-7). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Polyclonal Rabbit Anti-Human Kappa Light Chains, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of plasma cells and related lymphoid cells containing kappa light chains, and it is a useful tool for the classification of patients with monoclonal gammopathies (2) and amyloidosis (3, 4). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of B cells (5-7). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Polyclonal light chains of type kappa isolated from pooled human sera.
Specificity
The antibody reacts with free kappa chains as well as kappa chains in intact immunoglobulin molecules. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
Specificity tests; staining with Coomassie Brilliant Blue:
Double immunodiffusion: No reaction with lambda or gamma chains is seen when using 15 µL of concentrated antibody against 15 µL of lambda IgG.
Crossed immunoelectrophoresis: Only kappa-related precipitates appear when using 12.5 µL concentrated antibody per square cm gel area against 2 µL human plasma or 2 µL of a pool of concentrated urines from patients with tubular proteinuria.
Specificity tests; staining with Coomassie Brilliant Blue:
Double immunodiffusion: No reaction with lambda or gamma chains is seen when using 15 µL of concentrated antibody against 15 µL of lambda IgG.
Crossed immunoelectrophoresis: Only kappa-related precipitates appear when using 12.5 µL concentrated antibody per square cm gel area against 2 µL human plasma or 2 µL of a pool of concentrated urines from patients with tubular proteinuria.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
References
- Peterson LC, Brown BA, Crosson JT, Mladenovic J. Application of the immunoperoxidase technic to bone marrow trephine biopsies in the classification of patients with monoclonal gammopathies. Am J Clin Pathol 1986;85:688-93.
- Strøm EH, Fogazzi GB, Banfi G, Pozzi C, Mihatsch MJ. Light chain deposition disease of the kidney. Morphological aspects in 24 patients. Virchows Arch 1994;425:271-80.
- Jessup E. Antigen retrieval techniques for the demonstration of immunoglobulin light chains in formalin-fixed, paraffin-wax embedded sections. UK NEQAS immunocytochemistry news. Winter 1994/95; Issue 4:12-6.
- Taylor CR, Burns J. The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody. J Clin Path 1974;27:14-20.
- Harris NL, Poppema S, Data RE. Demonstration of immunoglobulin in malignant lymphomas. Use of an immunoperoxidase technic on frozen sections. Am J Clin Pathol 1982;78:14-21.
- Marshall-Taylor CE, Cartun RW, Mandich D, DiGuiseppe JA. Immunohistochemical detection of immunoglobulin light chain expression in B-cell non-Hodgkin lymphomas using formalin-fixed, paraffin-embedded tissues and a heat-induced epitope retrieval technique. Appl Immunohistochem Mol Morphol 2002;10:258-62.
Check Your Price & Order Online
| Product | Size | | IS506 RTU, FLEX
| 30 tests, 6 mL Product No. IS50630-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IS600) Positive tissue: Tonsil | 60 tests, 12 mL Product No. IS60061-2 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021-2 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |