Clone MAC 387
Reacts with a human cytoplasmic antigen (L1-antigen or calprotectin) which contains two different subunits (L1H and L1L). The protein is a member of the S100 family, and the subunits are in this context termed S100A8 and S100A9. It is expressed in granulocytes, blood monocytes, tissue histiocytes, squamous mucosal epithelia, and reactive epidermis. The antibody is useful for demonstrating tissue histiocytes in tissue sections from malignant lymphomas, and for detecting lymphoid neoplasms of histiocytic origin.
- Fagerhol MK. Nomenclature for proteins: is calprotectin a proper name for the elusive myelomonocytic protein? J Clin Pathol: Mol Pathol 1996;49:M74-9.
- Schäfer BW, Heizmann CW. The S100 family of EF-hand calciumbinding proteins: functions and pathology. Trends Biochem Sci 1996;21:134-40.
- Excellent specificity
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- Optimized for immunohistochemistry (IHC) with validated protocols
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For in vitro diagnostic use.
Monoclonal Mouse Anti-Human Myeloid/Histiocyte Antigen, Clone MAC 387, is intended for use in immunocytochemistry. The antibody labels tissue histiocytes and is a useful tool for the identification of histiocytosis belonging to either the malignant variety (1, 2) or the haemophagocytic syndromes (1). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Peripheral blood monocyte components isolated by affinity chromatography of detergent-solubilized monocyte material (2).
The antibody was clustered as anti-myeloid at the Third International Workshop and Conference on Human Leucocyte Differentiation Antigens (6).
In Western blotting of purified L1 protein separated by isoelectric focusing, the antibody labels bands identical to the bands labelled by polyclonal rabbit anti-L1 (5).
In air-dried, acetone-fixed cytocentrifuge preparations of peripheral blood, the antibody labels monocytes and granulocytes, whereas no labelling of lymphocytes is observed (2).
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: MAC 387 (2). Isotype: IgG1, kappa.
Mouse IgG concentration mg/L: See label on vial.
- Brandtzaeg P, Dale I, Gabrielsen T-Ø. The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function. Histopathology 1992;21:191-6.
- Flavell DJ, Jones DB, Wright DH. Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J Histochem Cytochem 1987;35:1217-26.
- Brandtzaeg P, Jones DB, Flavell DJ, Fagerhold MK. Mac 387 antibody and detection of formalin resistant myelomonocytic L1 antigen. J Clin Pathol 1988;41:963-70.
- Jones DB, Flavell DJ, Wright DH. M4.12. Tissue reactivity of a monoclonal antibody directed against cells of the human monocyte-macrophage lineage in formalin-fixed, paraffin-embedded tissue. In: McMichael AJ, Beverley PCL, Cobbold S, Crumpton MJ, Gilks W, Gotch FM, et al., editors. Leucocyte typing III. White cell differentiation antigens. Proceedings of the 3rd International Workshop and Conference
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Synonym(s) for Antigen
calgranulin, calprotectin, cystic fibrosis antigen, L1 antigen, MRP8/MRP14, S100A8/S100A9