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Monoclonal Mouse Anti-Human
p27Kip1
Clone SX53G8
p27Kip1, a cyclin-dependent kinase (cdk) inhibitor, regulates progression from G1 into S phase of the cell cycle by binding and inhibiting cyclin/cdks (1, 2). p27Kip1 exhibits structural and functional similarities with p21WAF1/Cip1 (1). Loss of the negative cell-cycle regulator, p27Kip1, may contribute to oncogenesis and tumor progression.
Isotype IgG1, kappa
- Formalin
- HIER
References
- Toyoshima H, Hunter T. p27, a novel inhibitor of G1 cyclin-cdk protein kinase activity, is related to p21. Cell 1994;78:67-74.
- Polyak K, Lee M-H, Erdjument-Bromage H, Koff A, Roberts JM, Tempst P, et al. Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals. Cell 1994;78:59-66.
Tonsil (FFPE) stained with Anti-p27Kip1, Code M7203.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human p27Kip1, Clone SX53G8, is intended for use in immunocytochemistry. The antibody labels cells expressing p27Kip1 and is a useful tool for investigation of breast, colon (1) and colorectal cancers (2). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human p27Kip1, Clone SX53G8, is intended for use in immunocytochemistry. The antibody labels cells expressing p27Kip1 and is a useful tool for investigation of breast, colon (1) and colorectal cancers (2). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Purified glutathione S-transferase (GST)-p27Kip1 fusion protein (1).
Specificity
In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1 (1).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: SX53G8 (1). Isotype: IgG1, kappa.
Mouse IgG concentration: See label on vial.
Clone: SX53G8 (1). Isotype: IgG1, kappa.
Mouse IgG concentration: See label on vial.
References
- Fredersdorf S, Burns J, Milne AM, Packham G, Fallis L, Gillett CE, et al. High level expression of p27Kip1 and cyclin D1 in some human breast cancer cells: Inverse correlation between the expression of p27Kip1 and degree of malignancy in human breast and colorectal cancers. Proc Natl Acad Sci 1997;94:6380-5.
- Schwandner O, Bruch H-P, Broll R. p21, p27, Cyclin D1, and p53 in rectal cancer: immunohistology with prognostic significance? Int J Colorectal Dis 2002;17:11-9.
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| Product | Size |  | M7203 Culture supernatant
| 1 mL Product No. M720301-2 |
|---|