p27Kip1, a cyclin-dependent kinase (cdk) inhibitor, regulates progression from G1 into S phase of the cell cycle by binding and inhibiting cyclin/cdks (1, 2). p27Kip1 exhibits structural and functional similarities with p21WAF1/Cip1 (1). Loss of the negative cell-cycle regulator, p27Kip1, may contribute to oncogenesis and tumor progression.
- Toyoshima H, Hunter T. p27, a novel inhibitor of G1 cyclin-cdk protein kinase activity, is related to p21. Cell 1994;78:67-74.
- Polyak K, Lee M-H, Erdjument-Bromage H, Koff A, Roberts JM, Tempst P, et al. Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals. Cell 1994;78:59-66.
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For in vitro diagnostic use.
Monoclonal Mouse Anti-Human p27Kip1, Clone SX53G8, is intended for use in immunocytochemistry. The antibody labels cells expressing p27Kip1 and is a useful tool for investigation of breast, colon (1) and colorectal cancers (2). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Purified glutathione S-transferase (GST)-p27Kip1 fusion protein (1).
In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1 (1).
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: SX53G8 (1). Isotype: IgG1, kappa.
Mouse IgG concentration: See label on vial.
- Fredersdorf S, Burns J, Milne AM, Packham G, Fallis L, Gillett CE, et al. High level expression of p27Kip1 and cyclin D1 in some human breast cancer cells: Inverse correlation between the expression of p27Kip1 and degree of malignancy in human breast and colorectal cancers. Proc Natl Acad Sci 1997;94:6380-5.
- Schwandner O, Bruch H-P, Broll R. p21, p27, Cyclin D1, and p53 in rectal cancer: immunohistology with prognostic significance? Int J Colorectal Dis 2002;17:11-9.
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