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Monoclonal Mouse Anti-
Proliferating Cell Nuclear Antigen
Clone PC10
The antibody reacts with a simple, linear epitope in proliferating cell nuclear antigen (PCNA). The PCNA is expressed by proliferating cells and reaches its maximum synthesis during the S-phase of the cell cycle.
Isotype IgG2a, kappa
- Frozen
- Formalin
- HIER
References
- Roos G, Landberg G, Huff JP, Houghten R, Takasaki Y, Tan EM. Analysis of the epitopes of proliferating cell nuclear antigen recognized by monoclonal antibodies. Lab Invest 1993;68:204-10.
- Bromley M, Rew D, Becciolini A, Balzi M, Chadwick C, Hewitt D, et al. A comparison of proliferation markers (BrdUrd, Ki-67, PCNA) determined at each cell position in the crypts of normal human colonic mucosa. Eur J Histochem 1996;40:89-100.
- Yu CC-W, Filipe M. Update on proliferation-associated antibodies applicable to formalin-fixed paraffin-embedded tissue and their application. Histochem J 1993;25:843-53.
Breast carcinoma (FFPE) stained with Anti-Proliferating Cell Nuclear Antigen.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Proliferating Cell Nuclear Antigen (PCNA), Clone PC10, is intended for use in immunocytochemistry. The antibody labels human cells expressing PCNA and may be a useful tool in determining proliferating cells in normal tissues and some forms of neoplasia (1). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Proliferating Cell Nuclear Antigen (PCNA), Clone PC10, is intended for use in immunocytochemistry. The antibody labels human cells expressing PCNA and may be a useful tool in determining proliferating cells in normal tissues and some forms of neoplasia (1). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Rat PCNA-protein A fusion protein obtained from vector pC2T (5).
Specificity
SDS-PAGE analysis of immunoprecipitates formed between the antibody and 35S-methionine-labelled extract of CV-1 cells (an immortalized line of monkey kidney epithelial cells) shows reaction primarily with a polypeptide corresponding to PCNA (5).
In Western blotting of cell extracts of HeLa cells, S. pompe (yeast) or S. frugiperda (insect) the antibody labels a band corresponding to PCNA (5).
In Western blotting of cell extracts of HeLa cells, S. pompe (yeast) or S. frugiperda (insect) the antibody labels a band corresponding to PCNA (5).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: PC10 (5). Isotype: IgG2a, kappa.
Mouse IgG concentration: see label on vial.
Clone: PC10 (5). Isotype: IgG2a, kappa.
Mouse IgG concentration: see label on vial.
References
- Hall PA, Levison DA, Woods AL, Yu CC-W, Kellock DB, Watkins JA, et al. Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 1990;162:285-94.
- Waseem NH, Lane DP. Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form. J Cell Sci 1990;96:121-9.
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| Product | Size |  | M0879 Culture supernatant
| 1 mL Product No. M087901-2 |
|---|
Synonym(s) for Antigen
PCNA