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Monoclonal Mouse Anti-Human
Prostatic Acid Phosphatase
Clone PASE/4LJ
Reacts with the glandular epithelium of normal and hyperplastic prostate, with carcinoma of the prostate, and with metastatic cells of prostate carcinoma. Occasionally, carcinoid tumors may stain positively.
Isotype IgG1, kappa
- Frozen
- Formalin
- HIER
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human Prostatic Acid Phosphatase, Clone PASE/4LJ, is intended for use in immunohistochemistry. The antibody labels glandular epithelium of the prostate and is a useful tool for the identification of normal prostatic tissue and primary and metastatic carcinoma of the prostate. Occasional samples of carcinoma of the bladder, rectal carcinoids, rare cases of other tumours, and macrophages may also be labelled by the antibody (1, 2). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human Prostatic Acid Phosphatase, Clone PASE/4LJ, is intended for use in immunohistochemistry. The antibody labels glandular epithelium of the prostate and is a useful tool for the identification of normal prostatic tissue and primary and metastatic carcinoma of the prostate. Occasional samples of carcinoma of the bladder, rectal carcinoids, rare cases of other tumours, and macrophages may also be labelled by the antibody (1, 2). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Purified prostatic acid phosphatase from human seminal plasma (1).
Specificity
In Western blotting of whole seminal plasma, the antibody labels a single band of 52 kDa corresponding to prostatic acid phophatase (1).
In ELISA the antibody specifically reacts with human semen excluding all other bodily excretions, including vaginal fluid, blood, saliva, female urine, nasal discharge, earwax, sweat and faeces. Additionally semen from dog, goat, pig, ox and sheep are negative, whereas the antibody cross-reacts with semen from non-human primates, including vervet monkey and chacma baboon (5).
As demonstrated by immunocytochemistry, the antibody shows no cross-reactivity with the prostatic acid phosphatase-equivalent protein in dog, rabbit, and rat (2).
In ELISA the antibody specifically reacts with human semen excluding all other bodily excretions, including vaginal fluid, blood, saliva, female urine, nasal discharge, earwax, sweat and faeces. Additionally semen from dog, goat, pig, ox and sheep are negative, whereas the antibody cross-reacts with semen from non-human primates, including vervet monkey and chacma baboon (5).
As demonstrated by immunocytochemistry, the antibody shows no cross-reactivity with the prostatic acid phosphatase-equivalent protein in dog, rabbit, and rat (2).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: PASE/4LJ (1). Isotype: IgG1, kappa.
Mouse IgG concentration: see label on vial.
Clone: PASE/4LJ (1). Isotype: IgG1, kappa.
Mouse IgG concentration: see label on vial.
References
- Haines AMR, Larkin SE, Heyderman E. A new monoclonal antibody to human prostatic acid phosphatase suitable for immunohistology in formalin-fixed paraffin-embedded tissue sections. Biochem Soc Trans 1987;15:1179-80.
- Haines AMR, Larkin SE, Richardson AP, Stirling RW, Heyderman E. A novel hybridoma antibody (PASE/4LJ) to human prostatic acid phosphatase suitable for immunohistochemistry. Br J Cancer 1989;60:887-92.
- Allen SM. An enzyme linked immunosorbent assay (ELISA) for detection of seminal fluid using a monoclonal antibody to prostatic acid phosphatase. J Immunoassay 1995;16:297-308.
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| Product | Size |  | M0792 Culture supernatant
| 1 mL Product No. M079201-2 |
|---|
Synonym(s) for Antigen
PSAP, PAP