For in vitro diagnostic use.

Truncated human WT1 protein corresponding to N-terminal amino acids 1-181 (1, 2).

Alternate splicing of the WT1 transcript gene results in four distinct mRNA species which are present in different amounts. The predicted molecular weight of the WT1 protein ranges from 46 to 49 kDa depending on the mRNA species however, the WT1 proteins derived from cells migrate at 50 to 55 kDa in SDS-PAGE (4). Monoclonal mouse anti-human WT1 (anti-WT1) recognizes an epitope found in the amino terminal 84 amino acids of WT1. Anti-WT1 reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2, frequently found in subsets of sporadic Wilms' tumors. In immunoprecipitation assays, anti-WT1 has been shown to recognize full-length and in vitro translated WT1. Anti-WT1 also detects full-length denatured WT1 (55 kDa) in Western blots of WT1 baculovirus-infected cell lysates (1).

Monoclonal mouse antibody supplied in liquid form as tissue culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris-HCl, pH 7.2, and 0.015 mol/L sodium azide.

Clone: 6F-H2 (1,2). Isotype: IgG1, kappa.

Mouse lgG concentration mg/L: See label on vial.

The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.

1. Rauscher JF, Morris JF, Fredericks WJ, Lopez-Guisa J, Balakrishnan C, Jost M, Herlyn M, Rodeck U. Characterization of monoclonal antibodies directed to the amino-terminus of the WT1, Wilms; tumor suppressor protein. Hybridoma 1998; 17:191
2. Menssen HD, et al. Presence of Wilms' tumor (wt1) transcripts and the WT1 nuclear protein in the majority of human acute leukemias. Leukemia 1995; 9:1060

4. Haber DA and Buckler AJ. WT1: A novel tumor suppressor gene inactivated in Wilms' tumor. New Biol 1992; 4(2):97

For in vitro diagnostic use.

FLEX Monoclonal Mouse Anti-Human Wilms' Tumor (WT1), Clone 6F-H2, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of Wilms tumor, acute leukemias, malignant mesothelioma, serous ovarian adenocarcinoma, granulosa cell tumor, and other tumors. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Truncated human WT1 protein corresponding to N-terminal amino acids 1-181 (1,2)

Alternate splicing of the WT1 transcript gene results in four distinct mRNA species which are present in different amounts. The predicted molecular weight of the WT1 protein ranges from 46 to 49 kDa depending on the mRNA species however, the WT1 proteins derived from cells migrate at 50 to 55 kDa in SDS-PAGE (4). This antibody recognizes an epitope found in the amino terminal 84 encoded amino acids of WT1. It reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2, frequently found in subsets of sporadic Wilms' tumors. In immunoprecipitation assays, the antibody has been shown to recognize full-length and in vitro translated WT1. It also detects full-length denatured WT1 (55 kDa) in Western blots of WT1 baculovirus-infected cell lysates (1).

Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN₃.

Clone: 6F-H2 (1,2). Isotype: IgG1, kappa.

1. Rauscher JF, et al. Submitted for publication.
2. Menssen HD, et al. Presence of Wilms' tumor (wt1) transcripts and the WT1 nuclear protein in the majority of human acute leukemias. Leukemia 1995; 9:1060

4. Haber DA and Buckler AJ. WT1: A novel tumor suppressor gene inactivated in Wilms' tumor. New Biol 1992; 4(2):97

For in vitro diagnostic use.

FLEX Monoclonal Mouse Anti-Human Wilms' Tumor (WT1), Clone 6F-H2, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of Wilms tumor, acute leukemias, malignant mesothelioma, serous ovarian adenocarcinoma, granulosa cell tumor, and other tumors. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Truncated human WT1 protein corresponding to N-terminal amino acids 1-181 (1,2)

Alternate splicing of the WT1 transcript gene results in four distinct mRNA species which are present in different amounts. The predicted molecular weight of the WT1 protein ranges from 46 to 49 kDa depending on the mRNA species however, the WT1 proteins derived from cells migrate at 50 to 55 kDa in SDS-PAGE (4). This antibody recognizes an epitope found in the amino terminal 84 encoded amino acids of WT1. It reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2, frequently found in subsets of sporadic Wilms' tumors. In immunoprecipitation assays, the antibody has been shown to recognize full-length and in vitro translated WT1. It also detects full-length denatured WT1 (55 kDa) in Western blots of WT1 baculovirus-infected cell lysates (1).

Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN₃.

Clone: 6F-H2 (1,2). Isotype: IgG1, kappa.

1. Rauscher JF, et al. Submitted for publication.
2. Menssen HD, et al. Presence of Wilms' tumor (wt1) transcripts and the WT1 nuclear protein in the majority of human acute leukemias. Leukemia 1995; 9:1060

4. Haber DA and Buckler AJ. WT1: A novel tumor suppressor gene inactivated in Wilms' tumor. New Biol 1992; 4(2):97