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Monoclonal Mouse Anti-Human
Podoplanin
Clone D2-40
Identifies the ~38 kDa O-linked transmembrane sialoglycoprotein podoplanin, which is expressed in the endothelium of lymphatic capillaries, but not in the blood vasculature (1). Besides the expression in lymphatic endothelium, podoplanin is also found in a variety of other tissues, including mesothelial cells, reticular cells, follicular dendritic cells, ovarian and testicular germ cells (2). Anti-Podoplanin, Clone D2-40 labels lymphatic endothelium whereas it is unreactive with vascular endothelium. In neoplastic tissue, immunostaining of lymphatic endothelium by the antibody has been shown to be useful in identifying lymphatic invasion of primary tumors.
Isotype IgG1, kappa
- Frozen
- Formalin
- HIER
References
- Breiteneder-Geleff S, Soleiman A, Kowalski H, Horvat R, Amann G, Kriehuber E, et al. Angiosarcomas express mixed endothelial phenotypes of blood and lymphatic capillaries: podoplanin as a specific marker for lymphatic endothelium. Am J Pathol 1999;154:385-94.
- Kalof AN and Cooper K. D2-40 Immunohistochemistry. Adv Anat Pathol 2009;16: 62-4.
Seminoma (FFPE) stained with FLEX Anti-Podoplanin, Code IR072/IS072.
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Seminoma. The neoplastic cells and lymphatic endothelial cells show a strong membranous and cytoplasmic staining reaction.
Clinical application for IR072/IS072. For identification of lymphatic invasion in a variety of cancers and the differential diagnosis between epithelioid mesothelioma and lung adenocarcinoma.
Mesothelioma. The majority of the neoplastic cells show a moderate to strong membranous and cytoplasmic staining reaction.
Clinical application for IR072/IS072. For identification of lymphatic invasion in a variety of cancers and the differential diagnosis between epithelioid mesothelioma and lung adenocarcinoma.
Appendix. Cajal cells show a moderate to strong cytoplasmic staining reaction. Endothelial cells should be negative.
Recommended control for IR072/IS072. Appendix/Tonsil.
Tonsil. The lymphatic endothelial cells and basal epithelial cells show a moderate to strong cytoplasmic staining reaction.
Recommended control for IR072/IS072. Appendix/Tonsil.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human Podoplanin, Clone D2-40 is intended for use in immunohistochemistry. This antibody labels the lymphatic endothelium marker podoplanin in normal and neoplastic tissues. The antibod may be useful in the identification of lymphatic invasion of a variety of cancers (1, 2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human Podoplanin, Clone D2-40 is intended for use in immunohistochemistry. This antibody labels the lymphatic endothelium marker podoplanin in normal and neoplastic tissues. The antibod may be useful in the identification of lymphatic invasion of a variety of cancers (1, 2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Dysgerminoma tissue (7).
Specificity
Monoclonal anti-D2-40 was tested by Western blotting of cell lysates from a variety of tumor cell lines. D2-40 identified a 40 kDa band in immunoblots of HEY ovarian epithelial carcinoma cells and a slightly higher molecular weight form in blots of MGH-U3 cells. D2-40 immunoreactivity has also been observed in Western blots of SK-OV-3 and Caov-3 ovarian epithelial carcinoma cells, PA-1PA-1 ovarian teratocarcinoma cells, Tera-1 and Tera-2 testicular teratocarcinoma cells, 833 embryonal carcinoma cells, RT-4 bladder transitional carcinoma cells and U-2 OS osteosarcoma cells. No reactivity was observed in Western blots of other tumor cell lines derived from a variety of cancers (1).
The effect of enzyme digestion on the immunoreactivity of D2-40 was tested using affinity purified antigen from HEY cells and intact HEY cells. The immunoreactivity of D2-40 with Western blots of purified antigen and with cells by flow cytometry was unaffected by the removal of sialic acid residues through neuraminidase treatment of the cells or protein. Treatment of protein or cells with O-sialoglycoprotease destroyed the immunoreactivity of D2-40 in both Western blotting and flow cytometry, demonstrating that the antigen recognized by D2-40 is an O-linked glycoprotein (1).
The effect of enzyme digestion on the immunoreactivity of D2-40 was tested using affinity purified antigen from HEY cells and intact HEY cells. The immunoreactivity of D2-40 with Western blots of purified antigen and with cells by flow cytometry was unaffected by the removal of sialic acid residues through neuraminidase treatment of the cells or protein. Treatment of protein or cells with O-sialoglycoprotease destroyed the immunoreactivity of D2-40 in both Western blotting and flow cytometry, demonstrating that the antigen recognized by D2-40 is an O-linked glycoprotein (1).
Reagent Provided
Monoclonal Mouse antibody provided in liquid form as tissue culture supernatant in 0.05 mol/L Tris-HCl, pH 7.2 and 0.015 mol/L sodium azide. This product contains stabilizing protein.
Clone: D2-40 (7). Isotype: IgG1, kappa.
Mouse lgG concentration mg/L: See label on vial.
M3619 may be used at a dilution 1:100-1:200 when performing IHC using the EnVision+™ detection system. These are guidelines only. Optimal antibody concentrations may vary depending on specimen and preparation method, and should be determined individually in each laboratory.
Clone: D2-40 (7). Isotype: IgG1, kappa.
Mouse lgG concentration mg/L: See label on vial.
M3619 may be used at a dilution 1:100-1:200 when performing IHC using the EnVision+™ detection system. These are guidelines only. Optimal antibody concentrations may vary depending on specimen and preparation method, and should be determined individually in each laboratory.
References
- Kahn HJ, Marks A. A new monoclonal antibody, D2-40, for detection of lymphatic invasion in primary tumors. Lab Invest 2002;82:1255-7.
- Kahn HJ, Bailey D, Marks A. Monoclonal antibody D2-40, a new marker of lymphatic endothelium, reacts with Kaposi's sarcoma and a subset of angiosarcomas. Mod Pathol 2002;15:434-40.
- Marks A, Sutherland DR, Bailey D, Iglesias J, Law J, Lei M, et al. Characterization and distribution of an oncofetal antigen (M2A antigen) expressed on testicular germ cell tumors. Br J Cancer 1999;80:569-78.
Check Your Price & Order Online
| Product | Size |  | M3619 Culture supernatant
| 0.2 mL Product No. M361929-2 |
|---|---|---|
| 1 mL Product No. M361901-2 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Podoplanin, Clone D2-40, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels the lymphatic endothelium marker podoplanin in normal and neoplastic tissues. The antibody may be useful in the identification of lymphatic invasion of a variety of cancers (1, 2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Podoplanin, Clone D2-40, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels the lymphatic endothelium marker podoplanin in normal and neoplastic tissues. The antibody may be useful in the identification of lymphatic invasion of a variety of cancers (1, 2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Dysgerminoma tissue (7).
Specificity
Anti-Podoplanin, Clone D2-40 was tested by Western blotting of cell lysates from a variety of tumor cell lines. The antibody identified a ~40 kDa band in immunoblots of HEY ovarian epithelial carcinoma cells and a slightly higher molecular weight form in blots of MGH-U3 cells. Immunoreactivity has also been observed in Western blots of SK-OV-3 and Caov-3 ovarian epithelial carcinoma cells, PA-1PA-1 ovarian teratocarcinoma cells, Tera-1 and Tera-2 testicular teratocarcinoma cells, 833 embryonal carcinoma cells, RT-4 bladder transitional carcinoma cells and U-2 OS osteosarcoma cells. No reactivity was observed in Western blots of other tumor cell lines derived from a variety of cancers (7).
The effect of enzyme digestion on the immunoreactivity of clone D2-40 was tested using affinity purified antigen from HEY cells and intact HEY cells. The immunoreactivity in Western blots of purified antigen and with cells by flow cytometry was unaffected by the removal of sialic acid residues through neuraminidase treatment of the cells or protein. Treatment of protein or cells with O-sialoglycoprotease destroyed the immunoreactivity of clone D2-40 in both Western blotting and flow cytometry, demonstrating that the antigen recognized is an O-linked glycoprotein (7).
The effect of enzyme digestion on the immunoreactivity of clone D2-40 was tested using affinity purified antigen from HEY cells and intact HEY cells. The immunoreactivity in Western blots of purified antigen and with cells by flow cytometry was unaffected by the removal of sialic acid residues through neuraminidase treatment of the cells or protein. Treatment of protein or cells with O-sialoglycoprotease destroyed the immunoreactivity of clone D2-40 in both Western blotting and flow cytometry, demonstrating that the antigen recognized is an O-linked glycoprotein (7).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: D2-40 (7). Isotype: IgG1, kappa.
Clone: D2-40 (7). Isotype: IgG1, kappa.
References
- Kahn HJ, Marks A. A new monoclonal antibody, D2-40, for detection of lymphatic invasion in primary tumors. Lab Invest 2002;82:1255-7.
- Kahn HJ, Bailey D, Marks A. Monoclonal antibody D2-40, a new marker of lymphatic endothelium, reacts with Kaposi's sarcoma and a subset of angiosarcomas. Mod Pathol 2002;15:434-40.
- Marks A, Sutherland DR, Bailey D, Iglesias J, Law J, Lei M, et al. Characterization and distribution of an oncofetal antigen (M2A antigen) expressed on testicular germ cell tumors. Br J Cancer 1999;80:569-78.
Check Your Price & Order Online
| Product | Size | | IR072 RTU, FLEX
| 60 tests, 12 mL Product No. IR07261-2 |
|---|
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human D2-40, Clone D2-40, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels the lymphatic endothelium marker podoplanin in normal and neoplastic tissues. The antibody may be useful in the identification of lymphatic invasion of a variety of cancers (1, 2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human D2-40, Clone D2-40, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels the lymphatic endothelium marker podoplanin in normal and neoplastic tissues. The antibody may be useful in the identification of lymphatic invasion of a variety of cancers (1, 2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Dysgerminoma tissue (7).
Specificity
Anti-Podoplanin, Clone D2-40 was tested by Western blotting of cell lysates from a variety of tumor cell lines. The antibody identified a ~40 kDa band in immunoblots of HEY ovarian epithelial carcinoma cells and a slightly higher molecular weight form in blots of MGH-U3 cells. Immunoreactivity has also been observed in Western blots of SK-OV-3 and Caov-3 ovarian epithelial carcinoma cells, PA-1PA-1 ovarian teratocarcinoma cells, Tera-1 and Tera-2 testicular teratocarcinoma cells, 833 embryonal carcinoma cells, RT-4 bladder transitional carcinoma cells and U-2 OS osteosarcoma cells. No reactivity was observed in Western blots of other tumor cell lines derived from a variety of cancers (1).
The effect of enzyme digestion on the immunoreactivity of clone D2-40 was tested using affinity purified antigen from HEY cells and intact HEY cells. The immunoreactivity in Western blots of purified antigen and with cells by flow cytometry was unaffected by the removal of sialic acid residues through neuraminidase treatment of the cells or protein. Treatment of protein or cells with O-sialoglycoprotease destroyed the immunoreactivity of clone D2-40 in both Western blotting and flow cytometry, demonstrating that the antigen recognized is an O-linked glycoprotein (1).
The effect of enzyme digestion on the immunoreactivity of clone D2-40 was tested using affinity purified antigen from HEY cells and intact HEY cells. The immunoreactivity in Western blots of purified antigen and with cells by flow cytometry was unaffected by the removal of sialic acid residues through neuraminidase treatment of the cells or protein. Treatment of protein or cells with O-sialoglycoprotease destroyed the immunoreactivity of clone D2-40 in both Western blotting and flow cytometry, demonstrating that the antigen recognized is an O-linked glycoprotein (1).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: D2-40 (7). Isotype: IgG1, kappa.
Clone: D2-40 (7). Isotype: IgG1, kappa.
References
- Kahn HJ, Marks A. A new monoclonal antibody, D2-40, for detection of lymphatic invasion in primary tumors. Lab Invest 2002;82:1255-7.
- Kahn HJ, Bailey D, Marks A. Monoclonal antibody D2-40, a new marker of lymphatic endothelium, reacts with Kaposi's sarcoma and a subset of angiosarcomas. Mod Pathol 2002;15:434-40.
- Marks A, Sutherland DR, Bailey D, Iglesias J, Law J, Lei M, et al. Characterization and distribution of an oncofetal antigen (M2A antigen) expressed on testicular germ cell tumors. Br J Cancer 1999;80:569-78.
Check Your Price & Order Online
| Product | Size | | IS072 RTU, FLEX
| 30 tests, 6 mL Product No. IS07230-2 |
|---|
Synonym(s) for Antigen
D2-40, lymphatic endothelium marker