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Monoclonal Mouse Anti-Human
EGFR-pY1197
Phosphorylation Site Specific
Clone DAK-H1-1197
Reacts with epidermal growth factor receptor (EGFR) phosphorylated at tyrosine residue 1197 (pY1197). Binding of epidermal growth factor to the extracellular domain of EGFR results in receptor dimerization and autophosphorylation on tyrosine residues, pY1197 being one of the major autophosphorylation sites (1, 2).
Isotype IgG2a
- Formalin
- HIER
References
- Chattopadhyay A, Vecchi M, Ji Q, Mernaugh R, Carpenter G. The role of individual SH2 domains in mediating association of phospholipase C-gamma1 with the activated EGF receptor. J Biol Chem 1999;274:26091-7.
- Lombardo CR, Consler TG, Kassel DB. In vitro phosphorylation of the epidermal growth factor receptor autophosphorylation domain by c-src: identification of phosphorylation sites and c-src SH2 domain binding sites. Biochem 1995;34:16456-66.
- Excellent specificity
- High lot to lot consistency
- For immunohistochemistry (IHC)
- Certified manufacturing facilities guarantee full quality control
Intended Use
For research use only. Not for use in diagnostic procedures.
Monoclonal Mouse Anti-Human EGFR-pY1197, Phosphorylation Site Specific, Clone DAK-H1-1197, is recommended for use in immunohistochemistry. The antibody labels human epidermal growth factor receptor only when, phosphorylated on tyrosine residue 1197 (EGFR-pY1197).
Monoclonal Mouse Anti-Human EGFR-pY1197, Phosphorylation Site Specific, Clone DAK-H1-1197, is recommended for use in immunohistochemistry. The antibody labels human epidermal growth factor receptor only when, phosphorylated on tyrosine residue 1197 (EGFR-pY1197).
Immunogen
Synthetic phosphopeptide containing residues surrounding tyrosine 1197 of human EGFR.
Specificity
In Western blotting of recombinant phosphorylated human HER1 (EGFR), HER2, HER3 or HER4 protein the antibody labels only the phosphorylated HER1 (EGFR) protein. The antibody does not label non-phosphorylated recombinant human HER1 (EGFR), HER2, HER3 or HER4.
When applied on formalin-fixed, paraffin-embedded cells transfected with either HER1 (EGFR), HER2, HER3 or HER4 phosphorylated intracellular domain constructs the antibody labels only cells expressing the HER1 (EGFR) intracellular domain.
Calf intestinal phosphatase (CIP) treatment of formalin-fixed, paraffin-embedded tissue resulted in loss of staining demonstrating that the antibody recognizes a phosphorylated antigen.
Liquid phase absorption with EGFR-pY1197 peptide prevented staining of formalin-fixed, paraffin embedded tissues. Similar absorption with non-phosphorylated EGFR-Y1197 peptide resulted in strong immunostaining of formalin-fixed, paraffin embedded tissues. This demonstrates that the antibody specifically recognizes the EGFR antigen only when it is phosphorylated on tyrosine 1197.
Database homology searching has shown potential cross-reactivity of the antibody to phosphorylated insulin receptor.
When applied on formalin-fixed, paraffin-embedded cells transfected with either HER1 (EGFR), HER2, HER3 or HER4 phosphorylated intracellular domain constructs the antibody labels only cells expressing the HER1 (EGFR) intracellular domain.
Calf intestinal phosphatase (CIP) treatment of formalin-fixed, paraffin-embedded tissue resulted in loss of staining demonstrating that the antibody recognizes a phosphorylated antigen.
Liquid phase absorption with EGFR-pY1197 peptide prevented staining of formalin-fixed, paraffin embedded tissues. Similar absorption with non-phosphorylated EGFR-Y1197 peptide resulted in strong immunostaining of formalin-fixed, paraffin embedded tissues. This demonstrates that the antibody specifically recognizes the EGFR antigen only when it is phosphorylated on tyrosine 1197.
Database homology searching has shown potential cross-reactivity of the antibody to phosphorylated insulin receptor.
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant (containing fetal bovine serum) dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 0.015 mol/L sodium azide.
Clone: DAK-H1-1197. Isotype: IgG2a, kappa.
Mouse Ig concentration mg/L: See label on vial.
Clone: DAK-H1-1197. Isotype: IgG2a, kappa.
Mouse Ig concentration mg/L: See label on vial.
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| Product | Size |  | M7299 Culture supernatant
| 0.2 mL Product No. M729929-8 |
|---|
Synonym(s) for Antigen
EGFR-pY1173, ptyr-1173 EGFR