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Monoclonal Mouse Anti-Human
Tissue Inhibitor of Metalloproteinases 1
Clone VT7
Reacts with tissue inhibitor of metalloproteinases 1 (TIMP-1). TIMP-1 is expressed in a proportion of stromal myofibroblast-like cells adjacent to invading cancer cells of colon adenocarcinomas and may be used to distinguish colon adenocarcinomas from colon adenomas (1). TIMP-1 is also expressed in neuroendocrine cells in normal and neoplastic tissues (2). TIMP-1 plays a pivotal role in extracellular matrix remodeling, and may affect malignant cell transformation, cell proliferation and apoptosis (3).
Isotype IgG1, kappa
- Formalin
- HIER
References
- Sørensen IV, Winther H, Foged NT, Fenger C, Brünner N. Tissue inhibitor of metalloproteinases 1 (TIMP-1) as an immunohistochemical marker for colorectal cancer. Eur J Cancer Supplements 2005;3:196.
- Sørensen IV, Fenger C, Winther H, Foged NT, Lademann U, Brünner N, et al. Characterization of anti-TIMP-1 monoclonal antibodies for immunohistochemical localization in formalin fixed paraffin embedded tissue. J Histochem Cytochem 2006;In press.
- Fassina G, Ferrari N, Brigati C, Benelli R, Santi L, Noonan DM, et al. Tissue inhibitors of metalloproteases: regulation and biological activities. Clin Exp Metastasis 2000;18:111-20.
Colon adenocarcinoma (FFPE) stained with Anti-TIMP-1, Code M7293.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human Tissue Inhibitor of Metalloproteinases 1, Clone VT7, is intended for use in immunohistochemistry. This antibody is useful for characterization of invasive colon adenocarcinomas, where TIMP-1 is expressed in a proportion of stromal myofibroblast-like cells adjacent to invading cancer cells (1), as well as for identification of neuroendocrine cells in normal and neoplastic tissues (2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human Tissue Inhibitor of Metalloproteinases 1, Clone VT7, is intended for use in immunohistochemistry. This antibody is useful for characterization of invasive colon adenocarcinomas, where TIMP-1 is expressed in a proportion of stromal myofibroblast-like cells adjacent to invading cancer cells (1), as well as for identification of neuroendocrine cells in normal and neoplastic tissues (2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Eukaryotic recombinant human TIMP-1 (r-h-TIMP-1) (6, 7).
Specificity
According to peptide-based linear epitope mapping, the antibody recognizes a linear sequence (LWTDQL) corresponding to amino acids 169-174 of mature TIMP-1 (6).
In Western blotting of both r-h-TIMP-1 and colorectal cancer patient plasma samples as well as culture supernatant from growing MCF7-S1 cells, the antibody labels two bands of 24-28 and 50-54 kDa, corresponding to differentially glycosylated monomer and homodimer TIMP-1, respectively (6).
In Western blotting of both r-h-TIMP-1 and colorectal cancer patient plasma samples as well as culture supernatant from growing MCF7-S1 cells, the antibody labels two bands of 24-28 and 50-54 kDa, corresponding to differentially glycosylated monomer and homodimer TIMP-1, respectively (6).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 0.015 mol/L sodium azide.
Clone: VT7. Isotype: IgG1, kappa.
Mouse IgG concentration mg/L: See label on vial.
Clone: VT7. Isotype: IgG1, kappa.
Mouse IgG concentration mg/L: See label on vial.
References
- Sørensen IV, Winther H, Foged NT, Fenger C and Brünner N. Tissue inhibitor of metalloproteinases 1 (TIMP-1) as an immunohistochemical marker for colorectal cancer. Eur J Cancer Supplements 2005;3:196.
- Sørensen IV, Fenger C, Winther H, Foged NT, Lademann U, Brünner N and Usher P. Characterization of anti-TIMP-1 monoclonal antibodies for immunohistochemical localization in formalin fixed paraffin embedded tissue. J Histochem Cytochem 2006; In press.
- Sørensen NM, Dowell BL, Stewart KD, Jensen V, Larsen L, Lademann U, et al. Establishment and characterization of 7 new monoclonal antibodies to tissue inhibitor of metalloproteinases-1. Tumour Biol 2005;26:71-80.
- Murphy G, Houbrechts A, Cockett MI, Williamson RA, Oshea M and Docherty AJP. The N-terminal domain of tissue inhibitor of metalloproteinases retains metalloproteinase inhibitory activity. Biochem 1991;30:8097-101.
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| Product | Size |  | M7293 Culture supernatant
| 0.2 mL Product No. M729329-2 |
|---|
Synonym(s) for Antigen
TIMP-1, TIMP1