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Polyclonal Rabbit Anti-Human
Alpha-1-Fetoprotein
A0008 can be used for detection of alpha-1-fetoprotein in human sera by enzyme-immunoassays (1). A0008 is also a valuable reagent for immunohistochemistry (2).
- Precipitation
- ELISA
- IHC
References
- MacDonal DJ, Kelly AM. The rapid quantitation of serum alpha-fetoprotein by two-site micro enzyme immunoassay. Clin Chim Acta 1978;87:367-72.
- Jacobsen GK, Jacobsen M, Clausen PP. Distribution of tumor-associated antigens in the various histiologic components of germ cell tumors of the testis. Am J Surg Pathol 1981;5:257-66.
- Excellent specificity and precipitation ability
- High lot to lot consistency
- Optimized for a variety of applications with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Polyclonal Rabbit Anti-Human Alpha-1-Fetoprotein, Code A0008, is intended for the determination of human alpha-1-fetoprotein (AFP) in immunohistochemistry (1-3), ELISA (4) and gel immunoprecipitation techniques.
Positive results may aid in the identification of non-neoplastic and neoplastic liver disease, yolk sac tumors and mixed germ cell tumors. However, it is important that the antibody is not used alone, but together with a panel of antibodies.
Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Polyclonal Rabbit Anti-Human Alpha-1-Fetoprotein, Code A0008, is intended for the determination of human alpha-1-fetoprotein (AFP) in immunohistochemistry (1-3), ELISA (4) and gel immunoprecipitation techniques.
Positive results may aid in the identification of non-neoplastic and neoplastic liver disease, yolk sac tumors and mixed germ cell tumors. However, it is important that the antibody is not used alone, but together with a panel of antibodies.
Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Immunogen
Alpha-1-fetoprotein isolated from human cord serum.
Specificity
The antibody reacts with human alpha-1-fetoprotein. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
The specificity of the antibody has been ascertained as follows:
Crossed immunoelectrophoresis: Only the alpha-1-fetoprotein precipitation arch appears when using 12.5 µL A0008 per cm2 gel area against 2 µL of a human cord serum fraction. No precipitation arch is seen when the antibody is tested against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
ELISA: In indirect ELISA no significant reaction is observed with normal human plasma.
As demonstrated by rocket immunoelectrophoresis, the antibody cross-reacts with the AFP-equivalent protein in cat and wildebeest (9).
The specificity of the antibody has been ascertained as follows:
Crossed immunoelectrophoresis: Only the alpha-1-fetoprotein precipitation arch appears when using 12.5 µL A0008 per cm2 gel area against 2 µL of a human cord serum fraction. No precipitation arch is seen when the antibody is tested against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
ELISA: In indirect ELISA no significant reaction is observed with normal human plasma.
As demonstrated by rocket immunoelectrophoresis, the antibody cross-reacts with the AFP-equivalent protein in cat and wildebeest (9).
Reagent Provided
Purified immunoglobulin fraction of rabbit antiserum in liquid form. In 0.1 mol/L NaCl, 15 mmol/L NaN3.
Protein concentration g/L: See label on vial.
The titre variation between different lots of A0008 is less than 10%. This is achieved by adjusting the titre of each individual lot to match the titre of an antibody reference preparation kept at -80 °C.
Protein concentration g/L: See label on vial.
The titre variation between different lots of A0008 is less than 10%. This is achieved by adjusting the titre of each individual lot to match the titre of an antibody reference preparation kept at -80 °C.
References
- Jacobsen GK, Jacobsen M. Alpha-fetoprotein (AFP) and human chorionic gonadotropin (HCG) in testicular germ cell tumours. A prospective immunohistochemical study. Acta Path Microbiol Immunol Scand Sect A 1983;91:165-76.
- Kodama T, Kameya T, Hirota T, Shimosato Y, Ohkura H, Mukojima T, et al. Production of alpha-fetoprotein, normal serum proteins, and human chorionic gonadotropin in stomach cancer: Histologic and immunohistochemical analyses of 35 cases. Cancer 1981;48:1647-55.
- Perrone T, Steeper TA, Dehner LP. Alpha-fetoprotein localization in pure ovarian teratoma. An immunohistochemical study of 12 cases. Am J Clin Pathol 1987;88:713-17.
- Hau J, Nilsson M, Skovgaard-Jensen H-J, de Souza A, Eriksen E, Wandall LT. Analysis of animal serum proteins using antisera against human analogous proteins. Scand J Lab Anim Sci 1990;17:3-7.
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| Product | Size |  | A0008 Ig fraction
| 0.2 mL Product No. A000829-2 |
|---|
Synonym(s) for Antigen
AFP