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Polyclonal Rabbit Anti-Human
IgA, Specific for Alpha-Chains
For use in methods demanding a very high specificity. The specificity and performance of the antibody have been ascertained in immunohistochemistry and ELISA. Additionally, the specificity has been tested by crossed immunoelectrophoresis using 12.5 microlitre antibody per square cm gel area against 2 microlitre human plasma. The antigen used for immunization is serum IgA.
- Blot
- ELISA
- IHC
- Excellent specificity and precipitation ability
- High lot to lot consistency
- Optimized for a variety of applications with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Polyclonal Rabbit Anti-Human IgA, Code A0262, is intended for use in immunohistochemistry. The antibody is also well suited for ELISA and immunoblotting (1).
In immunohistochemistry, the antibody labels plasma cells and related lymphoid cells containing IgA, and it is a useful tool for the classification of patients with B-cell neoplasias (2-4). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of plasma cells (2, 5). Differential identification is aided by the results from a panel of antibodies.Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Polyclonal Rabbit Anti-Human IgA, Code A0262, is intended for use in immunohistochemistry. The antibody is also well suited for ELISA and immunoblotting (1).
In immunohistochemistry, the antibody labels plasma cells and related lymphoid cells containing IgA, and it is a useful tool for the classification of patients with B-cell neoplasias (2-4). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of plasma cells (2, 5). Differential identification is aided by the results from a panel of antibodies.Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Immunogen
IgA isolated from a pool of normal human sera.
Specificity
The antibody reacts with the alpha-chains of human IgA. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
The specificity of the antibody has been ascertained as follows:
Crossed immunoelectrophoresis: Only the IgA immunoprecipitation arch appears when using 12.5 µL antibody cm2 gel area against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
ELISA: No significant reaction is seen in indirect ELISA using human IgG and IgM as coating antigens. In double antibody sandwich ELISA no significant reaction is observed with human plasma stripped of IgA.
Cross-reaction with IgA from other species may occur. As demonstrated by rocket immunoelectrophoresis, the antibody cross-reacts with the IgA-equivalent protein in cow, deer, goat, horse, mink, mouse, polecat, sheep and swine (9).
The specificity of the antibody has been ascertained as follows:
Crossed immunoelectrophoresis: Only the IgA immunoprecipitation arch appears when using 12.5 µL antibody cm2 gel area against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
ELISA: No significant reaction is seen in indirect ELISA using human IgG and IgM as coating antigens. In double antibody sandwich ELISA no significant reaction is observed with human plasma stripped of IgA.
Cross-reaction with IgA from other species may occur. As demonstrated by rocket immunoelectrophoresis, the antibody cross-reacts with the IgA-equivalent protein in cow, deer, goat, horse, mink, mouse, polecat, sheep and swine (9).
Reagent Provided
Purified immunoglobulin fraction of rabbit antiserum provided in liquid form. In 0.1 mol/L NaCl, 15 mmol/L NaN3.
Protein concentration g/L: See label on vial. Antibody titre (SRI): 2200 mg/L (8).
The titre variation between different lots of A0262 is less than 10%. This is achieved by adjusting the titre of each individual lot to match the titre of an antibody reference preparation kept at -80 °C.
Protein concentration g/L: See label on vial. Antibody titre (SRI): 2200 mg/L (8).
The titre variation between different lots of A0262 is less than 10%. This is achieved by adjusting the titre of each individual lot to match the titre of an antibody reference preparation kept at -80 °C.
References
- Withold W, Reinauer H. An immunoblotting procedure following agarose gel electrophoresis for detection of Bence Jones proteinuria compared with immunofixation and quantitative light chain determination. Eur J Clin Chem Clin Biochem 1995;33:135-8.
- Taylor CR, Burns J. The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody. J Clin Pathol 1974;27:14-20.
- Taylor CR, Mason DY. The immunohistological detection of intracellular immunoglobulin in formalin-paraffin sections from multiple myeloma and related conditions using the immunoperoxidase technique. Clin exp Immunol 1974;18:417-29.
- Taylor CR, Russell R, Chandor S. An immunohistologic study of multiple myeloma and related conditions, using an immunoperoxidase method. Am J Clin Pathol 1978;70:612-22.
- Beschorner R, Horny H-P, Petrusch UR, Kaiserling E. Frequent expression of haemopoietic and non-haemopoietic antigens by reactive plasma cells: an immunohistochemical study using formalin-fixed, paraffin-embedded tissue. Histol Histopathol 1999;14:805-12.
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| Product | Size |  | A0262 Ig fraction
| 1 mL Product No. A026201-2 | | F0204 FITC. Ig fraction
| 2 mL Product No. F020402-2 |  | F0316 FITC. F(ab')2
| 1 mL Product No. F031601-1 |
|---|