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Monoclonal Mouse Anti-Human
Cytokeratin, High Molecular Weight
Clone 34βE12
Reacts with the 68 kDa, 58 kDa, 56.5 kDa and 50 kDa proteins corresponding to cytokeratins 1, 5, 10 and 14.
The antibody labels squamous, ductal and complex epithelia, and is useful in the differentiation of benign prostate glands from prostatic adenocarcinoma and the classification of neoplastic tissue as carcinoma or epithelial origin.
Prostate adenocarcinoma (FFPE) stained with FLEX Anti-Cytokeratin, High Molecular Weight, Code IR051/IS051.
Prostate adenocarcinoma. Various staining reaction patterns are seen: Continuous cytoplasmic staining in normal gland, discontinuous pattern in PIN and no staining in invasive cancer cells.
Clinical application for IR051/IS051. For differential diagnosis between prostatic adenocarcinoma and prostatic intraepithelial neoplasia (PIN) or its benign mimics such as atypical hyperplasia.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
FLEX Monoclonal Mouse Anti-Human Cytokeratin, High Molecular Weight, Clone 34βE12, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of basal cells and squamous epithelium in various tissues. Positive results aid in the differentiation of benign prostate glands from prostatic adenocarcinoma and the classification of neoplastic tissue as carcinoma or epithelial origin. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Shah et al. (5) suggested clinical usefulness of anti-CK HMW, 34βE12 as an aid in the differentiation of Paget's disease and Bowen's disease (carcinoma in situ) from Pagetoid superficial spreading melanoma. Anti-CK HMW, 34βE12 positivity was reported in Paget's disease of the breast (5/5) and Bowen's disease (10/10), although 25% (1/4) of Paget's disease of the vulva and 0/6 of the Pagetoid superficial spreading melanomas stained (5).
Clone: 34βE12 (1). Isotype: IgG1, kappa
- Gown AM, Vogel AM. Monoclonal antibodies to intermediate filament proteins of human cells: Unique and cross-reacting antibodies. J Cell Biol 1982; 95:414.
- Moll R, Franke WW, Schiller DL. The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982; 31:11.
- Miettinen M. Keratin immunohistochemistry: update of applications and pitfalls. Pathol Ann 1993; 28:113
- Gown AM, Vogel AM. Monoclonal antibodies to human intermediate filament proteins II. Distribution of filaments in normal human tissues. Amer J Pathol 1984; 114:309.
- Shah KD, Tabibzadeh SS, Gerger MA. Immunohistochemical distinction of Paget's Disease from Bowen's Disease and superficial spreading melanoma with the use of monoclonal cytokeratin antibodies. Amer J Clin Pathol 1987; 88:689.
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| Product | Size |  | IR051 Clone 34βE12
| 60 tests, 12 mL Product No. IR05161-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Squamous epithelial cells | 120 tests, 24 mL Product No. IR75066-2 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021-2 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821-2 |
| Glass Slides | Silanized Slides (Code S3003) Recommended for greater adherence Alternative is FLEX IHC Microscope Slides (Code K8020) | 100 slides Product No. S300330-2 |