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Monoclonal Mouse Anti-Human
Cytokeratin 5/6
Clone D5/16 B4
Reacts strongly with cytokeratins 5 and 6 and weakly with cytokeratin 4. The antibody does not cross-react with cytokeratins 1, 7, 8, 10, 13, 14, 18 and 19.
It labels mesothelioma, and epithelial basal cells in prostate and tonsil. No reactivity with other mesodermally derived tissues, such as muscle and connective tissues, has been observed. For optimal results on formalin-fixed, paraffin-embedded specimens, heat-induced epitope retrieval using high pH buffer is recommended.
Cytokeratin 5/6 is expressed at low levels in adenocarcinomas, whereas it is present at high levels in epithelioid mesotheliomas, and the antibody is, therefore, very useful in the differentiation between these types of cancer.
Prostate hyperplasia and carcinoma (FFPE) stained with FLEX Anti-Cytokeratin 5/6, Code IR780/IS780.
Prostate hyperplasia and prostate carcinoma. The normal and benign glands show a distinct cytoplasmic staining reaction in the basal cells.
Clinical application for IR780/IS780. For differential diagnosis of poorly differentiated squamous cell carcinoma vs. adenocarcinoma, epithelioid mesothelioma vs. lung carcinoma, ductal hyperplasia vs. ductal carcinoma in situ of the breast, and prostate carcinoma vs. its benign mimics.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
FLEX Monoclonal Mouse Anti-Human Cytokeratin 5/6, Clone D5/16 B4, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the distinction between low differentiated squamous cell carcinoma and adenocarcinoma. Furthermore, the expression of CK5 by mesothelium makes this antibody valuable in the differentiation between epithelioid mesothelioma and lung carcinoma (1) when used together with other antibodies against mesothelioma markers, such as calretinin and thrombomodulin (2). Anti-CK 5/6 has also been found useful in the differential diagnosis of atypical proliferations of the breast (3). Antibodies to CK5/6 may also be useful as an aid in the differentiation between benign and malignant prostate glands (4). Differential identification is aided by the results from a panel of antibodies. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Clone: D5/16 B4. Isotype: IgG1, kappa.
- Clover J, Oates J, Edwards C. Anti-cytokeratin 5/6: a positive marker for epithelioid mesothelioma. Histopathology 1997; 31:140-3.
- Cury PM, Butcher DN, Fisher C, Corrin B, Nicholson AG. Value of the mesothelium-associated antibodies thrombomodulin, cytokeratin 5/6, calretinin, and CD44H in distinguishing epithelioid pleural mesothelioma from adenocarcinoma metastatic to the pleura. Mod Pathol 2000; 13:107-12.
- Otterbach F, Bankfalvi A, Bergner S, Decker T, Krech R, Boecker W. Cytokeratin 5/6 immunohistochemistry assists the differential diagnosis of atypical proliferations of the breast. Histopathology 2000; 37:232-40.
- Abrahams NA, Ormsby AH, Brainard J. Validation of cytokeratin 5/6 as an effective substitute for keratin 903 in the differentiation of benign from malignant glands in prostate needle biopsies. Histopathology 2002; 41:35-41.
- Moll R, Franke WW, Schiller DL. The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982; 31:11-24.
- Moll R, Löwe A, Laufer J, Franke WW. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol 1992; 140:427-47.
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| Product | Size |  | IR780 Clone D5/16 B4
| 60 tests, 12 mL Product No. IR78061-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Tonsil and prostate | 120 tests, 24 mL Product No. IR75066-2 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021-2 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |