For in vitro diagnostic use.

FLEX Monoclonal Mouse Anti-Human MUM1 protein, Clone MUM1p, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels the MUM1 protein, which is expressed in a subset of B cells in the light zone of the germinal centre (representing late stages of B-cell differentiation), plasma cells, activated T cells, and a wide spectrum of related haematolymphoid neoplasms. Of non-haematolymphoid neoplasms only a proportion of melanomas are labeled. The antibody is of value, for example, for the subclassification of lymphoid malignancies (1, 2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Recombinant MUM1 fusion protein (1).

Western blotting of pHeBo-CMV-MUM1-HA-transfected HeLa cell lysate, the antibody labels a band of 52 kDa corresponding to MUM1-HA protein, whereas no labeling is observed in non-transfected HeLa cell lysate. In Western blotting of lysates of IM9 (myeloma cell line), unfractionated tonsil cell suspensions, and PHA-stimulated T cells, the antibody labels a band of 50 kDa corresponding to MUM1 protein. No labeling is observed with lysates of U937 cells (myeloid cell line), and unstimulated T cells (1).

SDS-PAGE analysis of immunoprecipitates formed between lysate of the IM9 myeloma cell line and the antibody shows reaction with a 50 kDa protein corresponding to MUM1 protein (1).

Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.

Clone: MUM1p (1). Isotype: IgG1, kappa.