Polyclonal Rabbit Anti-Human

Chorionic Gonadotropin (hCG)

The isolated β-chain of hCG is used for immunization. The antibody cross-reacts with human luteinizing hormone (LH).

For immunohistochemical use, the cross-reaction with LH will not cause misinterpretation, and the antibody is well-suited for the demonstration of hCG in trophoblastic elements of germ cell tumors.

Chorionic carcinoma (FFPE) stained with FLEX Anti-hCG, Code IR508/IS508.

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Chorionic carcinoma. The neoplastic syncytiotrophoblasts show a moderate to strong cytoplasmic staining reaction with minimal background staining.

Clinical application for IR508/IS508. For identification of human chorionic gonadotropin-containing trophoblastic elements in germ cell tumors, choriocarcinoma, granulosa cell tumor, hydatiform mole and seminoma.

Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.

  • Optimized staining performance of both high- and low-expression structures
  • Crisp and clear staining with no background
  • Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy

Intended Use
For in vitro diagnostic use.

FLEX Polyclonal Rabbit Anti-Human Chorionic Gonadotropin, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of hCG-containing cells (1) and may be used for the demonstration of trophoblastic elements, e.g. in germ cell tumors (2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
The isolated beta-chain of hCG.
Specificity
The antibody reacts with hCG, and like most other polyclonal antibodies to beta-hCG it also shows a slight cross-reaction (about 4%) with LH (3). Traces of contaminating antibodies to human plasma proteins have been removed by solid-phase ab¬sorption.

In crossed immunoelectrophoresis only the hCG precipitation arch appears when using 12.5 µL Dako Code A0231 per cm2 gel area against 10 µL of concentrated urine from pregnant women (15th-16th week of gestation). No precipitation is seen when 12.5 µL concentrated antibody per cm2 gel area is tested against 10 µL of normal human plasma. Staining: Coomassie Brilliant Blue.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
References
  1. Morrish DW, Marusyk H, Siy O. Demonstration of specific secre¬tory granules for human chorionic gonadotropin in placenta. J Histochem Cytochem 1987;35:93-101.
  2. Jacobsen GK, Jacobsen M. Alpha-fetoprotein (AFP) and human chorionic gonadotropin (HCG) in testicular germ cell tumors. A prospective immunohistochemical study. Acta Path Microbiol Immunol Scand Sect A 1983;91:165-76.
  3. Mehta HC, MacDonald DJ. A sensitive enzyme immunoassay specific for human chorionic gonadotrophin. Clin Chim Acta 1982;121:245-50.
ProductSize
For In Vitro Diagnostic UseIS508
Specification Sheet
Safety Data Sheet:
Non-hazard classification statement
30 tests, 6 mL
Product No. IS50830-2

RECOMMENDED COMPANION PRODUCTS

StepProductSize
Pre-treatmentEnVision™ FLEX Target Retrieval Solution, High pH (Code K8004)
3-in-1 procedure using PT Link
3 x 30 mL
Product No. K800421-2
Antibody Dilution
Ready-to-use / Prediluted
 
Negative ControlFLEX Negative Control, Rabbit (Code IS600)
Positive tissue: Placenta
60 tests, 12 mL
Product No. IS60061-2
Visualization SystemEnVision™ FLEX (Code K8010)
Use High pH Target Retrieval Solution
400-600 tests
Product No. K801021-2
CounterstainHematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018)
Non-aqueous, permanent mounting medium is required
10 x 13 mL, 400-600 tests
Product No. K801821-2
Glass SlidesFLEX IHC Microscope Slides (Code K8020)
Recommended for greater adherence
5 x 100 slides
Product No. K802021-2

Synonym(s) for Antigen

hCG

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