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Monoclonal Mouse Anti-Human
Melan-A
Clone A103
Melan-A, isolated as a melanoma-specific antigen, is a transmembrane protein, which is expressed in skin, retina and the majority of cultured melanocytes as well as in melanomas and angiomyolipomas.
The antibody is useful for the identification of melanomas, and, if melanoma is ruled out, for adrenocortical carcinomas. Melan-A is also a useful marker for identification of angiomyolipomas.
References
- Fetsch PA, Cormier J, Hijazi YM. Immunocytochemical detection of MART-1 in fresh and paraffin-embedded malignant melanomas. J Immunother 1997;20:60-4.
- Kawakami Y, Eliyahu S, Delgado CH, Robbins PF, Sakaguchi K, Apella E, et al. Identification of a human melanoma antigen recognized by tumor-infiltrating lymphocytes associated with in vivo tumor rejection. Proc Natl Acad Sci USA 1994;91:6458-62.
- Jungbluth AA, Busam KJ, Gerald WL, Stockert E, Coplan KA, Iversen K, et al. A103: an anti-melan-A monoclonal antibody for the detection of malignant melanoma in paraffin-embedded tissues. Am J Surg Pathol 1998;5:595-602.
Melanoma (FFPE) stained with FLEX Anti-Melan-A, Code IR633/IS633.
Melanoma. The metastatic melanoma cells show a strong cytoplasmic staining reaction for melan-A.
Clinical application for IR633/IS633. For identification of melanoma. Melan-A is also expressed by various tumors such as granulosa cell tumor, adrenocortical carcinoma and angiomyolipoma.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Melan-A, Clone A103, Ready-to-Use, (Dako Autostainer/ Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of melanomas (1, 2), and, if melanoma is ruled out, for adrenocortical carcinomas (3, 4). The antibody is also applicable as a useful marker for angiomyolipomas (5). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Melan-A, Clone A103, Ready-to-Use, (Dako Autostainer/ Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of melanomas (1, 2), and, if melanoma is ruled out, for adrenocortical carcinomas (3, 4). The antibody is also applicable as a useful marker for angiomyolipomas (5). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Recombinant protein expressed in E. coli corresponding to Melan-A (1).
Specificity
In Western blotting of cell lysates from Melan-A mRNA positive cell lines, the antibody labels a protein doublet of 20-22 kDa, whereas no labeling is detected in Melan-A mRNA-negative cell lines (1).
In immunoprecipitates of Melan-A positive cell lines SK-MEL-13 and SK-MEL-19, the antibody labels a protein doublet of 20-22 kDa corresponding to Melan-A, whereas no precipitation is detected in Melan-A mRNA-negative melanoma cell lines (1).
In immunoprecipitates of Melan-A positive cell lines SK-MEL-13 and SK-MEL-19, the antibody labels a protein doublet of 20-22 kDa corresponding to Melan-A, whereas no precipitation is detected in Melan-A mRNA-negative melanoma cell lines (1).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
Clone: A103. Isotype: IgG1, kappa.
Clone: A103. Isotype: IgG1, kappa.
References
- Chen Y-T, Stockert E, Jungbluth A, Tsang S, Coplan KA, Scanlan MJ, et al. Serological analysis of Melan-A (MART-1), a melanocyte-specific protein homogeneously expressed in human melanomas. Proc Natl Acad Sci 1996;93:5915-9.
- Jungbluth AA, Busam KJ, Gerald WL, Stockert E, Coplan KA, Iversen K, et al. An anti-Melan-A monoclonal antibody for the detection of malignant melanoma in paraffin-embedded tissues. Am J Surg Pathol 1998;22:595-602.
- Busam KJ, Iversen K, Coplan KA, Old LJ, Stockert E, Chen Y-T, et al. Immunoreactivity for A103, an antibody to Melan-A (Mart-1), in adrenocortical and other steroid tumors. Am J Surg Pathol 1998;22:57-63.
- Loy TS, Phillips RW, Linder CL. A103 immunostaining in the diagnosis of adrenal cortical tumors. Arch Pathol Lab Med 2002;126:170-2.
- Jungbluth AA, Iversen K, Coplan K, Williamson B, Chen Y-T, Stockert T, et al. Expression of melanocyte-associated markers gp-100 and Melan-A/MART-1 in angiomyolipomas. Virchows Arch 1999;434:429-35.
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| Product | Size |  | IS633 Clone A103
| 30 tests, 6 mL Product No. IS63330-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Normal skin and malignant melanoma | 60 tests, 12 mL Product No. IS75061-2 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021-2 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |
Synonym(s) for Antigen
MART-1