For in vitro diagnostic use.

FLEX Monoclonal Mouse Anti-Human Neuron-Specific Enolase, Clone BBS/NC/VI-H14, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels both normal and neoplastic cells of neuronal and neuroendocrine origin (1), and although neuron-specific enolase (NSE) is not an exclusive neuronal marker, it may be used for the identification of peripheral nerves, neural and neuroendocrine tumors, such as neuroblastomas, retinoblastomas, desmoplastic malignant melanoma and small-cell lung cancer (2, 3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

γγ-enolase purified from human brain by a dissociation-reassociation method in a chromatofocusing column (1).

In Western blotting of reduced, SDS-denatured purified human γγ-enolase, αα-enolase, and extract of human muscle containing ββ-enolase, the antibody labels solely the γ-enolase subunit (1).

As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections, the antibody cross-reacts with the NSE-equivalent protein in pig (1).

Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.

Clone: BBS/NC/VI-H14 (1). Isotype: IgG1, kappa.