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Polyclonal Rabbit Anti-
S100
Reacts strongly with human S100B, and weakly or very weakly with S100A1 and S100A6, respectively. S100 from ox brain has been used for the immunization.
The antibody is a useful tool for the identification of S100-positive neoplasms, such as malignant melanoma (1), chondroblastoma, and schwannoma. Additionally, it is useful for the classification of tumors of suspected histiocytic/dendritic cell type (2). It is mandatory that antibodies to S100 are used together with a panel of other antibodies. Antibodies against S100 are useful in the classification of undifferentiated tumors of melanocytic origin (3).
References
- Orchard GE. Comparison of immunohistochemical labelling of melanocyte differentiation antibodies melan-A, tyrosinase and HMB 45 with NKIC3 and S100 protein in the evaluation of benign naevi and malignant melanoma. Histochem J 2000;32:475-81.
- Pileri SA, Grogan TM, Harris NL, Banks P, Campo E, Chan JKC, et al. (review). Tumours of histiocytes and accessory dendritic cells: an immunohistochemical approach to classification from the International Lymphoma Study Group based on 61 cases. Histopathology 2002;41:1-29.
- Bahrami A, Truong LD, Ro JA. Undifferentiated tumor: true identity by immunohistochemistry. Arch Pathol Lab Med 2008;132:326-48.
Schwannoma stained with FLEX Anti-S100, Code IR504/IS504.
Schwannoma. Most neoplastic cells show a strong nuclear and cytoplasmic staining reaction
Clinical application for IR504/IS504. Primarily for initial screening of melanoma. S100 protein is found in many tumors with neuroectodermal origin such as schwannoma and neurofibromas.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-S100, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels cells expressing S100 and is useful for the identification of S100-positive neoplasms, such as malignant melanoma (1, 2), Langerhans' histiocytosis (3), chondroblastoma (4), and schwannoma (5). A panel of antibodies including Polyclonal Anti-S100, Code Z0311, has been applied by the International Lymphoma Study Group for the classification of tumors of suspected histiocytic/dendritic cell type (6). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Polyclonal Rabbit Anti-S100, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels cells expressing S100 and is useful for the identification of S100-positive neoplasms, such as malignant melanoma (1, 2), Langerhans' histiocytosis (3), chondroblastoma (4), and schwannoma (5). A panel of antibodies including Polyclonal Anti-S100, Code Z0311, has been applied by the International Lymphoma Study Group for the classification of tumors of suspected histiocytic/dendritic cell type (6). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
S100 isolated from cow brain.
Specificity
The antibody has been solid-phase absorbed with human plasma and cow serum proteins.
In Western blotting of purified human recombinant S100 proteins, the antibody labels S100B strongly, S100A1 weakly, and S100A6 very weakly. No reaction was observed with the other S100 proteins tested, S100A2, S100A3 and S100A4 (8).
In indirect ELISA, the antibody shows no reaction with human plasma and cow serum.
As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections, the antibody cross-reacts with the S100 equivalent protein in man, cat, horse, mouse, rat, and swine.
In Western blotting of purified human recombinant S100 proteins, the antibody labels S100B strongly, S100A1 weakly, and S100A6 very weakly. No reaction was observed with the other S100 proteins tested, S100A2, S100A3 and S100A4 (8).
In indirect ELISA, the antibody shows no reaction with human plasma and cow serum.
As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections, the antibody cross-reacts with the S100 equivalent protein in man, cat, horse, mouse, rat, and swine.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
References
- Orchard GE. Comparison of immunohistochemical labeling of melanocyte differentiation antibodies melan-A, tyrosinase and HMB 45 with NKIC3 and S100 protein in the evaluation of benign naevi and malignant melanoma. Histochem J 2000; 32:475-81.
- Wick MR, Swanson PE, Rocamora A. Recognition of malignant melanoma by monoclonal antibody HMB-45. An immunohistochemical study of 200 paraffin-embedded cutaneous tumors. J Cutan Pathol 1988; 15:201-7.
- Ye F, Huang S-W, Dong H-J. Histiocytosis X. S-100 protein, peanut agglutinin, and transmission electron microscopy study. Am J Clin Pathol 1990; 94:627-31.
- Edel G, Ueda Y, Nakanishi J, Brinker KH, Roessner A, Blasius S, et al. Chondroblastoma in bone. A clinical, radiological, light and immunohistochemical study. Virchows Arch A Pathol Anat 1992; 421:355-66.
- Gould VE, Moll R, Moll I, Lee I, Schwechheimer K, Franke WW. The intermediate filament complement of the spectrum of nerve sheath neoplasms. Lab Invest 1986; 55:463-74.
- Pileri SA, Grogan TM, Harris NL, Banks P, Campo E, Chan JKC, et al. (review). tumors of histiocytes and accessory dendritic cells: an immunohistochemical approach to classification from the International Lymphoma Study Group based on 61 cases. Histopathology 2002; 41:1-29.
- Ilg EC, Schäfer BW, Heizmann CW. Expression pattern of S100 calcium-binding proteins in human tumors. Int J Cancer 1996; 68:325-32.
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| Product | Size |  | IS504
| 30 tests, 6 mL Product No. IS50430-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IS600) Positive tissue: Colon | 60 tests, 12 mL Product No. IS60061-2 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021-2 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |