For in vitro diagnostic use.

FLEX Monoclonal Mouse Anti-Human Mast Cell Tryptase, Clone AA1, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels human mast cells and is useful for the identification of very atypical or immature mast cells (MC) in mast cell leukemia, and for the detection of small, even minute, dense focal MC infiltrates in staging procedures in patients with known cutaneous mastocytosis (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Mast cell tryptase isolated from human lung (3).

In Western blotting of a tryptase-rich extract of human lung under reducing conditions, the antibody labels a band of approximately 32.5 kDa corresponding to human mast cell tryptase (3).

In indirect ELISA the antibody reacts with purified human mast cell tryptase (3).

Antibody-binding to the mast cell tryptase does not affect enzyme activity (3).

Periodate treatment of mast cell tryptase has no effect on antibody labeling, indicating that carbohydrate epitopes are not involved in antibody recognition. Nor is antibody recognition affected by the presence of heparin (3).

Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.

Clone: AA1 (3). Isotype: IgG1, kappa.