Monoclonal Mouse Anti-Human

ZAP-70

Clone 2F3.2

Reacts with ZAP-70 expressed in T cells, natural killer cells, pro/pre B cells but not in normal mature B cells.

The antibody is useful for the identification of a subset of chronic lymphocytic leukemias (CLL). In CLL, ZAP-70 expression is closely associated with an unmutated configuration of the immunoglobulin heavy-chain variable region (IgVH) genes. Identification of ZAP-70 expression may be beneficial for the differentiation between the Ig-unmutated CLL and Ig-mutated CLL (1, 2).

* This product is for in vitro diagnostic use only. The product embodies technology described in US Patent 7,329,502 and pending Canadian Patent Application No. 2,413,475.

References
  1. Carreras J, Villamor N, Colomo L, Moreno C, Cajal S, Crespo M, et al. Immunohistochemical analysis of ZAP70 expression in B-cell lymphoid neoplasms. J Pathol 2005;205;507-13.
  2. Wiestner A, Rosenwald A, Barry TS, Wright G, Davis RE, Henrickson SE, et al. ZAP-70 expression identifies a chronic lymphocytic leukemia subtype with unmutated immunoglobulin genes, inferior clinical outcome, and distinct gene expression profile. Blood 2003;101:4944-51.

B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma (FFPE) stained with FLEX Anti-ZAP-70, Code IR653/IS653.

+

B-CLL with reaction associated with mutated Ig heavy chain status. The neoplastic cells are negative, and only the T cells show a strong nuclear and cytoplasmic staining reaction.

Clinical application for IR653/IS653 For identification of ZAP-70 in a subset of B-cell chronic lymphocytic leukemias/small lymphocytic lymphoma.

Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.

  • Optimized staining performance of both high- and low-expression structures
  • Crisp and clear staining with no background
  • Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy

Intended Use
For in vitro diagnostic use.

FLEX Monoclonal Mouse Anti-Human ZAP-70, Clone 2F3.2, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for identification of ZAP-70 in a subset of chronic lymphocytic leukemias (1, 2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP-70 (7).
Specificity
In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP-70 (2). In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP-70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples (2). In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70 kDa protein (6). In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed (6).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.

Clone: 2F3.2. Isotype: IgG2a, kappa.
References
  1. Carreras J, Villamor N, Colomo L, Moreno C, Cajal S, Crespo M, et al. Immunohistochemical analysis of ZAP70 expression in B-cell lymphoid neoplasms. J Pathol 2005;205;507-13.
  2. Wiestner A, Rosenwald A, Barry TS, Wright G, Davis RE, Henrickson SE, et al. ZAP-70 expression identifies a chronic lymphocytic leukemia subtype with unmutated immunoglobulin genes, inferior clinical outcome, and distinct gene expression profile. Blood 2003;101:4944-51.
  1. Sup SJ, Domiati-Saad R, Kelley TW, Steinle R, Zhao X, Hsi ED. ZAP-70 expression in B-cell hematologic malignancy is not limited to CLL/SLL. Am J Clin Pathol 2004;122:582-7.
  2. Iwashima M, Irving BA, van Oers NSC, Chan AC, Weiss A. Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases. Science 1994;263:1136-9.
ProductSize
For In Vitro Diagnostic UseIS653 Clone 2F3.2
Specification Sheet
Safety Data Sheet:
Non-hazard classification statement
30 tests, 6 mL
Product No. IS65330-2

RECOMMENDED COMPANION PRODUCTS

StepProductSize
Pre-treatmentEnVision™ FLEX Target Retrieval Solution, High pH (Code K8004)
3-in-1 procedure using PT Link
3 x 30 mL
Product No. K800421-2
Antibody Dilution
Ready-to-use / Prediluted
 
Negative ControlFLEX Negative Control, Mouse (Code IS750)
Positive tissue: Tonsil
60 tests, 12 mL
Product No. IS75061-2
Visualization SystemEnVision™ FLEX (Code K8010)
Use High pH Target Retrieval Solution
400-600 tests
Product No. K801021-2
CounterstainHematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018)
Non-aqueous, permanent mounting medium is required
10 x 13 mL, 400-600 tests
Product No. K801821-2
Glass SlidesFLEX IHC Microscope Slides (Code K8020)
Recommended for greater adherence
5 x 100 slides
Product No. K802021-2

Country selection

Your Dako Account indicates that you are based in , you will be transfered to the web site for

Basket emptied

Your basket was emptied because of the country change.

Country selection

United States of America appears to be your location. To view country specific information on the Dako web site, please accept or change country below.



Get in Touch with Dako in United States of America

  • Pathology Products, Flow Cytometry Products and Specific Proteins
    Dako North America, Inc.

    general@dako.com
    +1 805 566 6655
    +1 805 566 6688

    6392 Via Real
    Carpinteria, CA 93013

  • Flow Cytometry Products
    Fisher Health Care

    +1 800 766 7000
    +1 800 926 1166

    9999 Veterans Memorial Drive
    Houston, TX 77038

Detailed contact information

Submit an Inquiry

How would you like us to follow up?


Cancel