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Monoclonal Rabbit Anti-Human

Cyclin D1

Clone EP12

This monoclonal rabbit antibody reacts with cyclin D1, a 36 kDa protein encoded by the CCND1 (bcl-1) gene located on chromosome 11q13. Cyclin D1 is part of the cell cycle regulation and oncogenic transformation in mammalian cells.

The cyclin-dependent kinases, CDK4 and CDK6, are associated with and activated by cyclin D1 thereby promoting G1 phase progression by retinoblastoma protein phosphorylating along with related proteins. Cyclin D1 is of interest in the investigation of tumorigenesis, as CCND1 amplification and/or cyclin D1 overexpression have been demonstrated in a variety of tumors. Antibodies to cyclin D1 are especially useful for identification of mantle cell lymphomas in the context of lymphoid tumors (1-3).

References
  1. De Boer CJ, Schuuring E, Dreef E, Peters G, Bartek J, Kluin PM, et al. Cyclin D1 protein analysis in the diagnosis of mantle cell lymphoma. Blood 1995;86:2715-23.
  2. Donnellan R, Chetty R. Cyclin D1 and human neoplasia. J Clin Pathol: Mol Pathol 1998;51:1-7.
  3. Falini B, Martelli MP, Tiacci E, Ascani S, Thiede C, Pileri SA. Immunohistochemical surrogates for genetic alterations of CCND1, PML, ALK, and NPM1 genes in lymphomas and acute myeloid leukemia. Best Practice & Research Clin Haematol 2010:23:417-31.

Mantle cell lymphoma (FFPE) stained with FLEX Anti-Cyclin D1, Code IR083/IS083.

+

Mantle cell lymphoma. The neoplastic cells show a moderate to strong nuclear staining reaction.

Clinical application for IR083/IS083. For identification of mantle cell lymphoma. Most B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma should be negative.

Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.

  • Optimized staining performance of both high- and low-expression structures
  • Crisp and clear staining with no background
  • Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy

Intended Use
For in vitro diagnostic use.

FLEX Monoclonal Rabbit Anti-Human Cyclin D1, Clone EP12, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. Antibodies to cyclin D1 are useful for the identification of mantle cell lymphomas (1-3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human cyclin D1.
Specificity
In Western blotting of MCF7 cells lysates the antibody labels a major band of approximately 36 kDa, corresponding to the expected molecular weight of cyclin D1.
Reagent Provided
Ready-to-use monoclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.

Clone: EP12.
References
  1. Donnellan R, Chetty R. Cyclin D1 and human neoplasia. J Clin Pathol: Mol Pathol 1998;51:1-7.
  2. De Boer CJ, Schuuring E, Dreef E, Peters G, Bartek J, Kluin PM, van Krieken HJM. Cyclin D1 protein analysis in the diagnosis of mantle cell lymphoma. Blood 1995;86:2715-23.
  3. Falini B, Martelli MP, Tiacci E, Ascani S, Thiede C, Pileri SA. Immunohistochemical surrogates for genetic alterations of CCDN1, PML, ALK, and NPM1 genes in lymphomas and acute myeloid leukemia. Best Practice &Research Clin Haematol 2010:23:417-31.
ProductSize
For In Vitro Diagnostic UseIS083 Clone EP12
Specification Sheet
Safety Data Sheet:
Non-hazard classification statement
30 tests, 6 mL
Product No. IS08330-2

RECOMMENDED COMPANION PRODUCTS

StepProductSize
Pre-treatmentEnVision™ FLEX Target Retrieval Solution, High pH (Code K8004)
3-in-1 procedure using PT Link
3 x 30 mL
Product No. K800421-2
Antibody Dilution
Ready-to-use / Prediluted
 
Negative ControlFLEX Negative Control, Rabbit (Code IS600)
Positive tissue: Tonsil
60 tests, 12 mL
Product No. IS60061-2
Visualization SystemEnVision™ FLEX (Code K8010)
Use High pH Target Retrieval Solution
400-600 tests
Product No. K801021-2
CounterstainHematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018)
Non-aqueous, permanent mounting medium is required
10 x 13 mL, 400-600 tests
Product No. K801821-2
Glass SlidesSilanized Slides (Code S3003)
Recommended for greater adherence
Alternative is FLEX IHC Microscope Slides (Code K8020)
100 slides
Product No. S300330-2

Synonym(s) for Antigen

Bcl-1, CCND1, PRAD-1

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